ID   URED_ECO57              Reviewed;         247 AA.
AC   Q8XAG3; Q7AFH7;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 3.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Putative urease accessory protein UreD homolog;
GN   Name=ureD1 {ECO:0000255|HAMAP-Rule:MF_01384};
GN   OrderedLocusNames=Z1142, ECs1321;
GN   and
GN   Name=ureD2 {ECO:0000255|HAMAP-Rule:MF_01384}; OrderedLocusNames=Z1581;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], TRANSCRIPTION, AND PREMATURE STOP CODON.
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=15470125; DOI=10.1099/mic.0.27280-0;
RA   Nakano M., Iida T., Honda T.;
RT   "Urease activity of enterohaemorrhagic Escherichia coli depends on a
RT   specific one-base substitution in ureD.";
RL   Microbiology 150:3483-3489(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01384}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01384}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01384}.
CC   -!- SIMILARITY: Belongs to the UreD family. {ECO:0000255|HAMAP-
CC       Rule:MF_01384}.
CC   -!- CAUTION: Could be the product of a pseudogene. Neither O157 strain
CC       expresses urease. In both O157 strains this CDS is shorter than in
CC       urease producing E.coli strains, due to a premature stop codon. Urease
CC       activity can be restored in O157 / Sakai upon complementation with a
CC       wild-type ureD. {ECO:0000305}.
CC   -!- CAUTION: This region of the chromosome is duplicated in strain O157:H7
CC       / EDL933 but not in O157:H7 / Sakai. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG55287.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAG55696.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE005174; AAG55287.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AE005174; AAG55696.1; ALT_INIT; Genomic_DNA.
DR   EMBL; BA000007; BAB34744.1; -; Genomic_DNA.
DR   PIR; A99794; A99794.
DR   PIR; D85654; D85654.
DR   RefSeq; NP_309348.1; NC_002695.1.
DR   AlphaFoldDB; Q8XAG3; -.
DR   SMR; Q8XAG3; -.
DR   STRING; 155864.EDL933_1041; -.
DR   EnsemblBacteria; AAG55287; AAG55287; Z1142.
DR   EnsemblBacteria; AAG55696; AAG55696; Z1581.
DR   EnsemblBacteria; BAB34744; BAB34744; ECs_1321.
DR   GeneID; 912366; -.
DR   KEGG; ece:Z1142; -.
DR   KEGG; ece:Z1581; -.
DR   KEGG; ecs:ECs_1321; -.
DR   PATRIC; fig|386585.9.peg.1426; -.
DR   eggNOG; COG0829; Bacteria.
DR   HOGENOM; CLU_056339_0_0_6; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   HAMAP; MF_01384; UreD; 1.
DR   InterPro; IPR002669; UreD.
DR   PANTHER; PTHR33643; PTHR33643; 1.
DR   Pfam; PF01774; UreD; 1.
PE   5: Uncertain;
KW   Chaperone; Cytoplasm; Nickel insertion; Reference proteome.
FT   CHAIN           1..247
FT                   /note="Putative urease accessory protein UreD homolog"
FT                   /id="PRO_0000340453"
SQ   SEQUENCE   247 AA;  27478 MW;  856382965492F85A CRC64;
     MLSTALPQNK KGWQAMLDLR FQRLHGKTTL TTRHHVGLLT VQRPFYPEEE TCHLCLLHPP
     GGIVGGDELI INAIIDSDCH TLITMPGASK FYRSSGAQAH LQQNLTLCNN ATLEWLPQDS
     IFFPGAHATL HTVFHLSSSS TLLAWDLLCL GRPVIGETFS HGTLANRLEI WVDGSPLLIE
     RLHVANGELT CVARKPWVGT MLFYLGNETQ LEDIREKLTP LENYAGATLT DGLLTVRFLS
     DDNLRCQ