URED_ECOLX
ID URED_ECOLX Reviewed; 278 AA.
AC Q03285;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 25-MAY-2022, entry version 54.
DE RecName: Full=Urease accessory protein UreD {ECO:0000255|HAMAP-Rule:MF_01384};
GN Name=ureD {ECO:0000255|HAMAP-Rule:MF_01384};
OS Escherichia coli.
OG Plasmid.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC STRAIN=1440 / UPEC;
RX PubMed=8449894; DOI=10.1128/jb.175.6.1860-1864.1993;
RA D'Orazio S.E., Collins C.M.;
RT "Characterization of a plasmid-encoded urease gene cluster found in members
RT of the family Enterobacteriaceae.";
RL J. Bacteriol. 175:1860-1864(1993).
CC -!- FUNCTION: Required for maturation of urease via the functional
CC incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC Rule:MF_01384}.
CC -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC hydrolysis-dependent molecular chaperone, activating the urease
CC apoprotein by helping to assemble the nickel containing metallocenter
CC of UreC. The UreE protein probably delivers the nickel.
CC {ECO:0000255|HAMAP-Rule:MF_01384}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01384}.
CC -!- INDUCTION: The probable operon is induced by urea.
CC {ECO:0000269|PubMed:8449894}.
CC -!- SIMILARITY: Belongs to the UreD family. {ECO:0000255|HAMAP-
CC Rule:MF_01384}.
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DR EMBL; L03307; AAA24744.1; -; Genomic_DNA.
DR PIR; A47090; A47090.
DR AlphaFoldDB; Q03285; -.
DR SMR; Q03285; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR HAMAP; MF_01384; UreD; 1.
DR InterPro; IPR002669; UreD.
DR PANTHER; PTHR33643; PTHR33643; 1.
DR Pfam; PF01774; UreD; 1.
PE 2: Evidence at transcript level;
KW Chaperone; Cytoplasm; Nickel insertion; Plasmid.
FT CHAIN 1..278
FT /note="Urease accessory protein UreD"
FT /id="PRO_0000067610"
SQ SEQUENCE 278 AA; 31413 MW; C024E444E13611B9 CRC64;
MSDFSGSGWL AEIFLRYELK RGVTRLTDKQ HIGPLMVQRP FYPEQGIAHT YLLHPPGGVV
GGDKLLINID VQPHAHALLT TPGATKFYRS AGGVARQVQT LTVAPNGFLE WLPQENIFFP
EAQVRLETHV RIASSSKFIS WEIQCLGRPV LNEQFDNGDI RGRLQFYIDD KLTLAESIFI
EGSQKQSAVM REFPMVGSLY IYPASDELKA ELHESLAVFF STEVRPLEYG LTDVDGILVL
RLLGSQTEPM MACFAHIWQA TRQYWLGYCP EPPRIWAT