ID   URED_MYCS2              Reviewed;         271 AA.
AC   A0QRF3; I7G4S5;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Urease accessory protein UreD {ECO:0000255|HAMAP-Rule:MF_01384};
GN   Name=ureD {ECO:0000255|HAMAP-Rule:MF_01384};
GN   OrderedLocusNames=MSMEG_1096, MSMEI_1064;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01384}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01384}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01384}.
CC   -!- SIMILARITY: Belongs to the UreD family. {ECO:0000255|HAMAP-
CC       Rule:MF_01384}.
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DR   EMBL; CP000480; ABK73647.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP37544.1; -; Genomic_DNA.
DR   RefSeq; WP_011727405.1; NZ_SIJM01000011.1.
DR   RefSeq; YP_885491.1; NC_008596.1.
DR   AlphaFoldDB; A0QRF3; -.
DR   SMR; A0QRF3; -.
DR   STRING; 246196.MSMEI_1064; -.
DR   EnsemblBacteria; ABK73647; ABK73647; MSMEG_1096.
DR   EnsemblBacteria; AFP37544; AFP37544; MSMEI_1064.
DR   GeneID; 66732559; -.
DR   KEGG; msg:MSMEI_1064; -.
DR   KEGG; msm:MSMEG_1096; -.
DR   PATRIC; fig|246196.19.peg.1084; -.
DR   eggNOG; COG0829; Bacteria.
DR   OrthoDB; 1741524at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   HAMAP; MF_01384; UreD; 1.
DR   InterPro; IPR002669; UreD.
DR   Pfam; PF01774; UreD; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion; Reference proteome.
FT   CHAIN           1..271
FT                   /note="Urease accessory protein UreD"
FT                   /id="PRO_0000346582"
SQ   SEQUENCE   271 AA;  28659 MW;  90D66B956769AC5B CRC64;
     MKLAERTAAD LRFARAGAHT VLTRRRHRWP FVIGRVFADP QDPGLGTLTL QNAAGTVIPG
     DVIRQRIEVV DGGRAAVNGQ GATLISGVPG GDASAEETEL YVDAHSWLRF DPAPRILTAH
     ARHRQRTEVC VAHRGCAVVV DAVVLHPDLD AATFGSIEST VTVRSPGGAL LAMDAQVLDA
     PPVTGRFTAF GTVYFVGAGF TGGHHEGPTD PPGVYTAVTE LPNGAGCAVR IAAADGGTLR
     TYLRLLSAET AFQRASTRTF TDGMPFRRSQ V