ID   URED_POLAQ              Reviewed;         291 AA.
AC   A4SY48;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 2.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Urease accessory protein UreD {ECO:0000255|HAMAP-Rule:MF_01384};
GN   Name=ureD {ECO:0000255|HAMAP-Rule:MF_01384}; OrderedLocusNames=Pnuc_1197;
OS   Polynucleobacter asymbioticus (strain DSM 18221 / CIP 109841 /
OS   QLW-P1DMWA-1) (Polynucleobacter necessarius subsp. asymbioticus).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Polynucleobacter.
OX   NCBI_TaxID=312153;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 18221 / CIP 109841 / QLW-P1DMWA-1;
RX   PubMed=22675600; DOI=10.4056/sigs.2395367;
RA   Meincke L., Copeland A., Lapidus A., Lucas S., Berry K.W., Del Rio T.G.,
RA   Hammon N., Dalin E., Tice H., Pitluck S., Richardson P., Bruce D.,
RA   Goodwin L., Han C., Tapia R., Detter J.C., Schmutz J., Brettin T.,
RA   Larimer F., Land M., Hauser L., Kyrpides N.C., Ivanova N., Goker M.,
RA   Woyke T., Wu Q.L., Pockl M., Hahn M.W., Klenk H.P.;
RT   "Complete genome sequence of Polynucleobacter necessarius subsp.
RT   asymbioticus type strain (QLW-P1DMWA-1(T)).";
RL   Stand. Genomic Sci. 6:74-83(2012).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01384}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01384}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01384}.
CC   -!- SIMILARITY: Belongs to the UreD family. {ECO:0000255|HAMAP-
CC       Rule:MF_01384}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABP34412.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000655; ABP34412.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_048812119.1; NC_009379.1.
DR   AlphaFoldDB; A4SY48; -.
DR   SMR; A4SY48; -.
DR   STRING; 312153.Pnuc_1197; -.
DR   EnsemblBacteria; ABP34412; ABP34412; Pnuc_1197.
DR   GeneID; 31481583; -.
DR   KEGG; pnu:Pnuc_1197; -.
DR   eggNOG; COG0829; Bacteria.
DR   HOGENOM; CLU_056339_0_0_4; -.
DR   Proteomes; UP000000231; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   HAMAP; MF_01384; UreD; 1.
DR   InterPro; IPR002669; UreD.
DR   PANTHER; PTHR33643; PTHR33643; 1.
DR   Pfam; PF01774; UreD; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion.
FT   CHAIN           1..291
FT                   /note="Urease accessory protein UreD"
FT                   /id="PRO_0000340473"
SQ   SEQUENCE   291 AA;  32094 MW;  42A7AA6D8F95019F CRC64;
     MFLAPDNAVK QLFRSLSPSW MAKLSLSYER TPIGTVLKKS LHEGPLRVQK ALYPEGDDIC
     HTVIIHPPAG IAGGDTLDIQ VAVGKGSHVV LSTPSATKWY KSFKNPATQN VQFELGENAK
     LDWLPQENLF FKGANSNLIT KLNLPASASF IGWDALMLGR HASGEEWSSG HIHLLNEIRR
     DGQLIWIENG HIDAEDPYSK SLPQLGSWPV CATLLALGPQ CSNHLAENLS EMMPWTNSIR
     AGVTLMPQGI VIVRAVSIDI EMARNFMIDV WSKLRPIIHG VPAQPLRLWA S