CAD1_ARATH
ID CAD1_ARATH Reviewed; 561 AA.
AC Q9C7N2; Q940R0;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=MACPF domain-containing protein CAD1;
DE AltName: Full=Protein CONSTITUTIVELY ACTIVATED CELL DEATH 1;
DE Short=Protein CAD1;
GN Name=CAD1; OrderedLocusNames=At1g29690; ORFNames=F15D2.24;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=15799997; DOI=10.1093/pcp/pci095;
RA Morita-Yamamuro C., Tsutsui T., Sato M., Yoshioka H., Tamaoki M., Ogawa D.,
RA Matsuura H., Yoshihara T., Ikeda A., Uyeda I., Yamaguchi J.;
RT "The Arabidopsis gene CAD1 controls programmed cell death in the plant
RT immune system and encodes a protein containing a MACPF domain.";
RL Plant Cell Physiol. 46:902-912(2005).
RN [6]
RP GENE FAMILY.
RX PubMed=16900325; DOI=10.1007/s11103-006-9001-6;
RA Noutoshi Y., Kuromori T., Wada T., Hirayama T., Kamiya A., Imura Y.,
RA Yasuda M., Nakashita H., Shirasu K., Shinozaki K.;
RT "Loss of Necrotic Spotted Lesions 1 associates with cell death and defense
RT responses in Arabidopsis thaliana.";
RL Plant Mol. Biol. 62:29-42(2006).
CC -!- FUNCTION: Negatively controls the salicylic acid (SA)-mediated pathway
CC of programmed cell death in plant immunity.
CC {ECO:0000269|PubMed:15799997}.
CC -!- TISSUE SPECIFICITY: Mainly expressed in the vascular system.
CC {ECO:0000269|PubMed:15799997}.
CC -!- DISRUPTION PHENOTYPE: Constitutively activated HR-like cell death
CC phenotype with endogenous accumulation of high levels of salicylic acid
CC (SA) and constitutively activated defense phenotype against challenge
CC with P.syringae. Dwarf plant with normal cotyledons, but dark brown- or
CC black-colored cell death lesions on the true leaves.
CC {ECO:0000269|PubMed:15799997}.
CC -!- SIMILARITY: Belongs to the complement C6/C7/C8/C9 (TC 1.C.39) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL06838.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC068667; AAG51760.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31118.1; -; Genomic_DNA.
DR EMBL; AY054177; AAL06838.1; ALT_FRAME; mRNA.
DR EMBL; AY074857; AAL75908.1; -; mRNA.
DR EMBL; BT030337; ABO38750.1; -; mRNA.
DR PIR; C86420; C86420.
DR RefSeq; NP_564333.1; NM_102709.4.
DR AlphaFoldDB; Q9C7N2; -.
DR BioGRID; 25081; 1.
DR STRING; 3702.AT1G29690.1; -.
DR TCDB; 1.C.39.11.3; the membrane attack complex/perforin (macpf) family.
DR iPTMnet; Q9C7N2; -.
DR PaxDb; Q9C7N2; -.
DR PRIDE; Q9C7N2; -.
DR ProteomicsDB; 240275; -.
DR EnsemblPlants; AT1G29690.1; AT1G29690.1; AT1G29690.
DR GeneID; 839846; -.
DR Gramene; AT1G29690.1; AT1G29690.1; AT1G29690.
DR KEGG; ath:AT1G29690; -.
DR Araport; AT1G29690; -.
DR TAIR; locus:2013673; AT1G29690.
DR eggNOG; ENOG502QRZ9; Eukaryota.
DR HOGENOM; CLU_034245_1_0_1; -.
DR InParanoid; Q9C7N2; -.
DR OMA; SPDVINM; -.
DR OrthoDB; 520445at2759; -.
DR PhylomeDB; Q9C7N2; -.
DR PRO; PR:Q9C7N2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C7N2; baseline and differential.
DR Genevisible; Q9C7N2; AT.
DR GO; GO:0008219; P:cell death; IMP:TAIR.
DR GO; GO:0006955; P:immune response; IMP:TAIR.
DR GO; GO:0009626; P:plant-type hypersensitive response; IMP:UniProtKB.
DR GO; GO:2000031; P:regulation of salicylic acid mediated signaling pathway; IEA:InterPro.
DR GO; GO:0010337; P:regulation of salicylic acid metabolic process; IMP:UniProtKB.
DR InterPro; IPR044663; CAD1/NSL1-like.
DR InterPro; IPR020864; MACPF.
DR PANTHER; PTHR33199; PTHR33199; 1.
DR Pfam; PF01823; MACPF; 1.
DR SMART; SM00457; MACPF; 1.
DR PROSITE; PS51412; MACPF_2; 1.
PE 2: Evidence at transcript level;
KW Hypersensitive response; Immunity; Innate immunity; Plant defense;
KW Reference proteome.
FT CHAIN 1..561
FT /note="MACPF domain-containing protein CAD1"
FT /id="PRO_0000415539"
FT DOMAIN 11..314
FT /note="MACPF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00745"
FT REGION 489..514
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 496..512
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 291
FT /note="R -> W (in Ref. 3; AAL06838)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 561 AA; 62223 MW; C45990E64D15B32D CRC64;
MENRKGGNFS VPSSEALTTT LRNAIQALGR GFDVTSDVRL LYCKGAPGSR LVRIEEGQNR
DLELSHGFLL PNVPADIDCS RGNSGTQRIS VCSFHEMAEE FNVRSGVKGN IPLGCFNAMF
NYTGSWQVDA ASTKSLALVG YFIPLYDVKL AKLTLVLHNE IRRAVPSSWD PASLASFIEN
YGTHIVTSVT IGGRDVVYIR QHQSSPLPVS EIENYVNDMI KHRFHEAESQ SITGPLKYKD
KDITVIFRRR GGDDLEQSHA RWAETVPAAP DIINMTFTPI VSLLEGVPGL RHLTRAIELY
LEYKPPIEDL QYFLDYQIAR AWAPEQSNLQ RKEPVCSSLQ FSLMGPKLFI SADQVTVGRK
PVTGLRLSLE GSKQNRLSIH LQHLVSLPKI LQPHWDSHVP IGAPKWQGPE EQDSRWFEPI
KWKNFSHVST SPIEHTETHI GDLSGVHIVT GAQLGVWDFG SKNVLHLKLL FSKVPGCTIR
RSVWDHTPVA SSGRLEPGGP STSSSTEEVS GQSGKLAKIV DSSEMLKGPQ DLPGHWLVTG
AKLGVEKGKI VLRVKYSLLN Y
