CAD1_DICDI
ID CAD1_DICDI Reviewed; 213 AA.
AC P54657; Q23855; Q54MQ4;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 25-MAY-2022, entry version 122.
DE RecName: Full=Calcium-dependent cell adhesion molecule 1;
DE Short=CAD-1;
DE Short=DdCAD-1;
DE AltName: Full=GP24;
GN Name=cadA; Synonyms=cad1; ORFNames=DDB_G0285793;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 43-62 AND 118-133,
RP FUNCTION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=8663243; DOI=10.1074/jbc.271.27.16399;
RA Wong E.F.S., Brar S.K., Sesaki H., Yang C., Siu C.-H.;
RT "Molecular cloning and characterization of DdCAD-1, a Ca2+-dependent cell-
RT cell adhesion molecule, in Dictyostelium discoideum.";
RL J. Biol. Chem. 271:16399-16408(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Yang C., Siu C.-H.;
RT "Cloning, regulation, and promoter analysis of the cadA gene in
RT Dictyostelium discoideum.";
RL Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 4-193.
RC STRAIN=AX2;
RA Winckler T.;
RT "Cloning of a putative 25 kDa protein from Dictyostelium discoideum.";
RL Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=AX2;
RX PubMed=16926386; DOI=10.1074/mcp.m600113-mcp200;
RA Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M.,
RA Soldati T.;
RT "Proteomics fingerprinting of phagosome maturation and evidence for the
RT role of a Galpha during uptake.";
RL Mol. Cell. Proteomics 5:2228-2243(2006).
RN [6]
RP INDUCTION.
RX PubMed=17481898; DOI=10.1016/j.cub.2007.04.029;
RA Bakthavatsalam D., Brazill D., Gomer R.H., Eichinger L., Rivero F.,
RA Noegel A.A.;
RT "A G protein-coupled receptor with a lipid kinase domain is involved in
RT cell-density sensing.";
RL Curr. Biol. 17:892-897(2007).
RN [7]
RP STRUCTURE BY NMR.
RX PubMed=17057715; DOI=10.1038/nsmb1162;
RA Lin Z., Sriskanthadevan S., Huang H., Siu C.-H., Yang D.;
RT "Solution structures of the adhesion molecule DdCAD-1 reveal new insights
RT into Ca(2+)-dependent cell-cell adhesion.";
RL Nat. Struct. Mol. Biol. 13:1016-1022(2006).
CC -!- FUNCTION: Mediates calcium-dependent cell-cell adhesion during the
CC early stage of development. {ECO:0000269|PubMed:8663243}.
CC -!- INTERACTION:
CC P54657; P54657: cadA; NbExp=2; IntAct=EBI-8446773, EBI-8446773;
CC P54657; P02599: calA; NbExp=4; IntAct=EBI-8446773, EBI-1808395;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:8663243}.
CC Note=Associated with the ecto-surface of the plasma membrane. May be
CC transported to the plasma membrane via contractile vacuoles and its
CC cell surface association may be mediated by an integral membrane
CC protein.
CC -!- DEVELOPMENTAL STAGE: Expressed soon after the initiation of
CC development. {ECO:0000269|PubMed:8663243}.
CC -!- INDUCTION: Expression is controlled by rpkA.
CC {ECO:0000269|PubMed:17481898}.
CC -!- DOMAIN: Cell binding activity is dependent on the N-terminal segment
CC whereas the C-terminal domain tethers the protein to the cell membrane.
CC -!- PTM: The N-terminus is blocked.
CC -!- SIMILARITY: Belongs to the Dictyostelium CAD family. {ECO:0000305}.
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DR EMBL; U49650; AAC47135.1; -; mRNA.
DR EMBL; AF340153; AAK17205.1; -; Genomic_DNA.
DR EMBL; AAFI02000079; EAL64543.1; -; Genomic_DNA.
DR EMBL; U20997; AAA62645.1; -; mRNA.
DR RefSeq; XP_638048.1; XM_632956.1.
DR PDB; 1YHP; NMR; -; A=2-213.
DR PDB; 2B1O; NMR; -; A=2-213.
DR PDBsum; 1YHP; -.
DR PDBsum; 2B1O; -.
DR AlphaFoldDB; P54657; -.
DR BMRB; P54657; -.
DR SMR; P54657; -.
DR BioGRID; 1250289; 1.
DR DIP; DIP-29248N; -.
DR IntAct; P54657; 1.
DR MINT; P54657; -.
DR STRING; 44689.DDB0191175; -.
DR PaxDb; P54657; -.
DR EnsemblProtists; EAL64543; EAL64543; DDB_G0285793.
DR GeneID; 8625286; -.
DR KEGG; ddi:DDB_G0285793; -.
DR dictyBase; DDB_G0285793; cadA.
DR eggNOG; ENOG502SGUC; Eukaryota.
DR HOGENOM; CLU_1362582_0_0_1; -.
DR InParanoid; P54657; -.
DR OMA; ESEIVCQ; -.
DR PhylomeDB; P54657; -.
DR EvolutionaryTrace; P54657; -.
DR PRO; PR:P54657; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005938; C:cell cortex; TAS:dictyBase.
DR GO; GO:0005911; C:cell-cell junction; IDA:dictyBase.
DR GO; GO:0000331; C:contractile vacuole; IDA:dictyBase.
DR GO; GO:0005737; C:cytoplasm; IDA:dictyBase.
DR GO; GO:0030139; C:endocytic vesicle; IDA:dictyBase.
DR GO; GO:0009897; C:external side of plasma membrane; IDA:dictyBase.
DR GO; GO:0031012; C:extracellular matrix; IDA:dictyBase.
DR GO; GO:0005576; C:extracellular region; IDA:dictyBase.
DR GO; GO:0030175; C:filopodium; IDA:dictyBase.
DR GO; GO:0030027; C:lamellipodium; IDA:dictyBase.
DR GO; GO:0005811; C:lipid droplet; HDA:dictyBase.
DR GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR GO; GO:0005886; C:plasma membrane; IDA:dictyBase.
DR GO; GO:0001726; C:ruffle; IDA:dictyBase.
DR GO; GO:0005509; F:calcium ion binding; IDA:dictyBase.
DR GO; GO:0005516; F:calmodulin binding; IPI:dictyBase.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0031152; P:aggregation involved in sorocarp development; TAS:dictyBase.
DR GO; GO:0051702; P:biological process involved in interaction with symbiont; IDA:dictyBase.
DR GO; GO:0016339; P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules; IDA:dictyBase.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IMP:dictyBase.
DR GO; GO:0007157; P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules; IDA:dictyBase.
DR GO; GO:0010468; P:regulation of gene expression; IDA:dictyBase.
DR GO; GO:1904643; P:response to curcumin; IDA:dictyBase.
DR GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR GO; GO:0031288; P:sorocarp morphogenesis; IMP:dictyBase.
DR Gene3D; 2.60.40.1720; -; 1.
DR InterPro; IPR015059; Ca_cell_adhesion_N_dom.
DR InterPro; IPR038423; CAD_C_sf.
DR InterPro; IPR011024; G_crystallin-like.
DR InterPro; IPR029283; Membrane-bd.
DR Pfam; PF08964; Crystall_3; 1.
DR Pfam; PF14564; Membrane_bind; 1.
DR SUPFAM; SSF49695; SSF49695; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Calcium; Cell adhesion; Cell membrane;
KW Direct protein sequencing; Membrane; Reference proteome; Repeat.
FT CHAIN 1..213
FT /note="Calcium-dependent cell adhesion molecule 1"
FT /id="PRO_0000089273"
FT REPEAT 1..48
FT /note="1"
FT REPEAT 49..97
FT /note="2"
FT REPEAT 98..146
FT /note="3"
FT REPEAT 147..194
FT /note="4"
FT REGION 1..194
FT /note="4 X approximate tandem repeats"
FT STRAND 8..13
FT /evidence="ECO:0007829|PDB:1YHP"
FT TURN 14..16
FT /evidence="ECO:0007829|PDB:1YHP"
FT STRAND 20..23
FT /evidence="ECO:0007829|PDB:1YHP"
FT STRAND 25..30
FT /evidence="ECO:0007829|PDB:1YHP"
FT HELIX 36..39
FT /evidence="ECO:0007829|PDB:1YHP"
FT STRAND 43..46
FT /evidence="ECO:0007829|PDB:1YHP"
FT STRAND 51..56
FT /evidence="ECO:0007829|PDB:1YHP"
FT STRAND 68..71
FT /evidence="ECO:0007829|PDB:1YHP"
FT STRAND 73..78
FT /evidence="ECO:0007829|PDB:1YHP"
FT HELIX 81..83
FT /evidence="ECO:0007829|PDB:1YHP"
FT STRAND 87..92
FT /evidence="ECO:0007829|PDB:1YHP"
FT STRAND 97..108
FT /evidence="ECO:0007829|PDB:1YHP"
FT STRAND 115..121
FT /evidence="ECO:0007829|PDB:1YHP"
FT STRAND 127..134
FT /evidence="ECO:0007829|PDB:1YHP"
FT STRAND 136..140
FT /evidence="ECO:0007829|PDB:1YHP"
FT STRAND 150..160
FT /evidence="ECO:0007829|PDB:1YHP"
FT STRAND 165..176
FT /evidence="ECO:0007829|PDB:1YHP"
FT TURN 177..180
FT /evidence="ECO:0007829|PDB:1YHP"
FT STRAND 181..185
FT /evidence="ECO:0007829|PDB:1YHP"
FT TURN 188..190
FT /evidence="ECO:0007829|PDB:1YHP"
FT STRAND 193..201
FT /evidence="ECO:0007829|PDB:1YHP"
FT STRAND 204..209
FT /evidence="ECO:0007829|PDB:1YHP"
SQ SEQUENCE 213 AA; 23926 MW; FEE35BA62236293C CRC64;
MSVDANKVKF FFGKNCTGES FEYNKGETVR FNNGDKWNDK FMSCLVGSNV RCNIWEHNEI
DTPTPGKFQE LAQGSTNNDL TSINGLSKFQ VLPGAFQWAV DVKIVNKVNS TAGSYEMTIT
PYQVDKVACK DGDDFVQLPI PKLTPPDSEI VSHLTVRQTH TPYDYVVNGS VYFKYSPTTG
QVTVIKKDET FPKNMTVTQD DNTSFIFNLN SEK
