ID   UREE1_BRUSU             Reviewed;         162 AA.
AC   Q8G2P7; G0KBX0;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Urease accessory protein UreE 1 {ECO:0000255|HAMAP-Rule:MF_00822};
GN   Name=ureE1 {ECO:0000255|HAMAP-Rule:MF_00822}; Synonyms=ureE;
GN   OrderedLocusNames=BR0271, BS1330_I0272;
OS   Brucella suis biovar 1 (strain 1330).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=204722;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=12271122; DOI=10.1073/pnas.192319099;
RA   Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F.,
RA   Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA   Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J.A., Van Aken S.E.,
RA   Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L.,
RA   Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.;
RT   "The Brucella suis genome reveals fundamental similarities between animal
RT   and plant pathogens and symbionts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=22038969; DOI=10.1128/jb.06181-11;
RA   Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.;
RT   "Revised genome sequence of Brucella suis 1330.";
RL   J. Bacteriol. 193:6410-6410(2011).
RN   [3]
RP   CHARACTERIZATION OF ROLE IN VIRULENCE, AND DISRUPTION PHENOTYPE.
RC   STRAIN=1330;
RX   PubMed=17578575; DOI=10.1186/1471-2180-7-57;
RA   Bandara A.B., Contreras A., Contreras-Rodriguez A., Martins A.M.,
RA   Dobrean V., Poff-Reichow S., Rajasekaran P., Sriranganathan N.,
RA   Schurig G.G., Boyle S.M.;
RT   "Brucella suis urease encoded by ure1 but not ure2 is necessary for
RT   intestinal infection of BALB/c mice.";
RL   BMC Microbiol. 7:57-57(2007).
CC   -!- FUNCTION: Involved in urease metallocenter assembly. Binds nickel.
CC       Probably functions as a nickel donor during metallocenter assembly.
CC       {ECO:0000255|HAMAP-Rule:MF_00822}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00822}.
CC   -!- DISRUPTION PHENOTYPE: Disrupting the ure1 operon causes loss of urease
CC       activity, decreased resistance to low pH killing in vitro and decreased
CC       pathogen survival when inoculated in BALB/c mice by gavage.
CC       {ECO:0000269|PubMed:17578575}.
CC   -!- SIMILARITY: Belongs to the UreE family. {ECO:0000255|HAMAP-
CC       Rule:MF_00822}.
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DR   EMBL; AE014291; AAN29220.1; -; Genomic_DNA.
DR   EMBL; CP002997; AEM17633.1; -; Genomic_DNA.
DR   RefSeq; WP_004690531.1; NZ_KN046804.1.
DR   AlphaFoldDB; Q8G2P7; -.
DR   SMR; Q8G2P7; -.
DR   EnsemblBacteria; AEM17633; AEM17633; BS1330_I0272.
DR   GeneID; 45051404; -.
DR   GeneID; 55590050; -.
DR   KEGG; bms:BR0271; -.
DR   KEGG; bsi:BS1330_I0272; -.
DR   PATRIC; fig|204722.21.peg.1754; -.
DR   HOGENOM; CLU_093757_1_0_5; -.
DR   OMA; AYDARCK; -.
DR   PhylomeDB; Q8G2P7; -.
DR   Proteomes; UP000007104; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006457; P:protein folding; IEA:InterPro.
DR   GO; GO:0065003; P:protein-containing complex assembly; IEA:InterPro.
DR   GO; GO:0019627; P:urea metabolic process; IEA:InterPro.
DR   CDD; cd00571; UreE; 1.
DR   HAMAP; MF_00822; UreE; 1.
DR   InterPro; IPR012406; UreE.
DR   InterPro; IPR007864; UreE_C_dom.
DR   InterPro; IPR004029; UreE_N.
DR   InterPro; IPR036118; UreE_N_sf.
DR   Pfam; PF05194; UreE_C; 1.
DR   Pfam; PF02814; UreE_N; 1.
DR   PIRSF; PIRSF036402; Ureas_acces_UreE; 1.
DR   SMART; SM00988; UreE_N; 1.
DR   SUPFAM; SSF69287; SSF69287; 1.
PE   1: Evidence at protein level;
KW   Chaperone; Cytoplasm; Nickel; Nickel insertion; Virulence.
FT   CHAIN           1..162
FT                   /note="Urease accessory protein UreE 1"
FT                   /id="PRO_0000223407"
FT   REGION          143..162
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   162 AA;  18481 MW;  C65F2F78A6F486FD CRC64;
     MFRAIAIIRA HEVIDAVPAS HIVLERDERH LRRKAITLEN GEKILADFAE PVVLEHGDRL
     VLDDGREIEI RAASEELYEI RGRDPRHIAE LAWHIGNRHL AAQIETDRIF ILRDHVIRVM
     LEGLGATVTD VVAIFSPLRG AYSGGHQHHH GHDHDHHHPD HE