ID   UREEF_BORBO             Reviewed;         433 AA.
AC   O06708;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Bifunctional urease accessory protein UreEF;
DE   Includes:
DE     RecName: Full=Urease accessory protein UreE;
DE   Includes:
DE     RecName: Full=Urease accessory protein UreF;
GN   Name=ureEF;
OS   Bordetella bronchiseptica (Alcaligenes bronchisepticus).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Alcaligenaceae; Bordetella.
OX   NCBI_TaxID=518;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BB7866;
RX   PubMed=9524276; DOI=10.1016/s0378-1119(97)00651-3;
RA   McMillan D.J., Mau M., Walker M.J.;
RT   "Characterisation of the urease gene cluster in Bordetella
RT   bronchiseptica.";
RL   Gene 208:243-251(1998).
CC   -!- FUNCTION: Involved in urease metallocenter assembly. Binds nickel.
CC       Probably functions as a nickel donor during metallocenter assembly (By
CC       similarity). {ECO:0000250}.
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000250}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the UreE family.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the UreF family.
CC       {ECO:0000305}.
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DR   EMBL; AF000579; AAC46129.1; -; Genomic_DNA.
DR   AlphaFoldDB; O06708; -.
DR   SMR; O06708; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003824; F:catalytic activity; IEA:UniProtKB-KW.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006457; P:protein folding; IEA:InterPro.
DR   GO; GO:0065003; P:protein-containing complex assembly; IEA:InterPro.
DR   GO; GO:0019627; P:urea metabolic process; IEA:InterPro.
DR   CDD; cd00571; UreE; 1.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_00822; UreE; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR012406; UreE.
DR   InterPro; IPR007864; UreE_C_dom.
DR   InterPro; IPR004029; UreE_N.
DR   InterPro; IPR036118; UreE_N_sf.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF05194; UreE_C; 1.
DR   Pfam; PF02814; UreE_N; 1.
DR   Pfam; PF01730; UreF; 1.
DR   SMART; SM00988; UreE_N; 1.
DR   SUPFAM; SSF69287; SSF69287; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Multifunctional enzyme; Nickel; Nickel insertion.
FT   CHAIN           1..433
FT                   /note="Bifunctional urease accessory protein UreEF"
FT                   /id="PRO_0000344203"
FT   REGION          1..200
FT                   /note="Urease accessory protein UreE"
FT   REGION          200..433
FT                   /note="Urease accessory protein UreF"
SQ   SEQUENCE   433 AA;  46704 MW;  09A8AFC91AF874D7 CRC64;
     MKIANTFIKR GAAGGLDLSQ APGVTLTLAE RRRSRQRLDL DEGRGELGMA IERGQTLRDG
     DVLVAEDGTY VVVRAALEDV ARVTAATPWQ LARAAYHLGN RHVLLEIAER HLQFEYDAVL
     IDMLAQLGGV TATRLRAVFE PDVGAYGGGH RHGHDESFGD DYALAQAAYH AHEHTPMRTP
     MPADTATFIP VMATAASTAS MTPSLDAGQL AALLHLSSPA LPIGGFSYSQ GLEAAIELGL
     VHDEASTLAW IESQLVTVMA RAEAPLWCLL FEAWRAGDDA AAHGWNQWFH ASRETRELRQ
     ETEQMGRSLA RLAQELGWGT AATRAAVAAL RPATLPAVHA CACAMWALPR EAGLGAYVFS
     WLENQVAAAI KGVPLGQMAG QRMLERLRAG LPAVLADARA RAGATPPRLD TFAPQYALVS
     ARHETQFSRL FRS