UREE_HELPY
ID UREE_HELPY Reviewed; 170 AA.
AC Q09064;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Urease accessory protein UreE {ECO:0000255|HAMAP-Rule:MF_00822};
GN Name=ureE {ECO:0000255|HAMAP-Rule:MF_00822}; OrderedLocusNames=HP_0070;
OS Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=85962;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=85P;
RX PubMed=1313413; DOI=10.1128/jb.174.8.2466-2473.1992;
RA Cussac V., Ferrero R.L., Labigne A.;
RT "Expression of Helicobacter pylori urease genes in Escherichia coli grown
RT under nitrogen-limiting conditions.";
RL J. Bacteriol. 174:2466-2473(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700392 / 26695;
RX PubMed=9252185; DOI=10.1038/41483;
RA Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT "The complete genome sequence of the gastric pathogen Helicobacter
RT pylori.";
RL Nature 388:539-547(1997).
RN [3]
RP REVIEW ON VIRULENCE OF H.PYLORI.
RX PubMed=17313591; DOI=10.1111/j.1574-6968.2007.00648.x;
RA Clyne M., Dolan B., Reeves E.P.;
RT "Bacterial factors that mediate colonization of the stomach and virulence
RT of Helicobacter pylori.";
RL FEMS Microbiol. Lett. 268:135-143(2007).
CC -!- FUNCTION: Involved in urease metallocenter assembly. Binds nickel.
CC Probably functions as a nickel donor during metallocenter assembly.
CC {ECO:0000255|HAMAP-Rule:MF_00822}.
CC -!- INTERACTION:
CC Q09064; Q09066: ureG; NbExp=3; IntAct=EBI-7742396, EBI-7742750;
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the UreE family. {ECO:0000255|HAMAP-
CC Rule:MF_00822}.
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DR EMBL; M84338; AAA25023.1; -; Genomic_DNA.
DR EMBL; AE000511; AAD07133.1; -; Genomic_DNA.
DR PIR; B41834; B41834.
DR RefSeq; NP_206870.1; NC_000915.1.
DR RefSeq; WP_000583097.1; NC_018939.1.
DR PDB; 3L9Z; X-ray; 2.08 A; A=1-170.
DR PDB; 3LA0; X-ray; 2.86 A; A/B/C/D=1-170.
DR PDB; 3NXZ; X-ray; 2.70 A; A/B/C/D=1-170.
DR PDB; 3NY0; X-ray; 3.09 A; A/B/C/D=1-170.
DR PDB; 3TJ8; X-ray; 1.59 A; A/B=1-170.
DR PDB; 3TJ9; X-ray; 2.52 A; A/B/C/D=1-170.
DR PDB; 3TJA; X-ray; 2.00 A; A/B/C/D=1-170.
DR PDBsum; 3L9Z; -.
DR PDBsum; 3LA0; -.
DR PDBsum; 3NXZ; -.
DR PDBsum; 3NY0; -.
DR PDBsum; 3TJ8; -.
DR PDBsum; 3TJ9; -.
DR PDBsum; 3TJA; -.
DR AlphaFoldDB; Q09064; -.
DR SMR; Q09064; -.
DR DIP; DIP-3132N; -.
DR IntAct; Q09064; 1.
DR MINT; Q09064; -.
DR STRING; 85962.C694_00335; -.
DR PaxDb; Q09064; -.
DR DNASU; 898943; -.
DR EnsemblBacteria; AAD07133; AAD07133; HP_0070.
DR KEGG; hpy:HP_0070; -.
DR PATRIC; fig|85962.47.peg.73; -.
DR eggNOG; COG2371; Bacteria.
DR OMA; YVDLEWF; -.
DR PhylomeDB; Q09064; -.
DR BioCyc; MetaCyc:HP_RS00355-MON; -.
DR EvolutionaryTrace; Q09064; -.
DR Proteomes; UP000000429; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051082; F:unfolded protein binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006457; P:protein folding; IEA:InterPro.
DR GO; GO:0065003; P:protein-containing complex assembly; IEA:InterPro.
DR GO; GO:0019627; P:urea metabolic process; IEA:InterPro.
DR CDD; cd00571; UreE; 1.
DR HAMAP; MF_00822; UreE; 1.
DR InterPro; IPR012406; UreE.
DR InterPro; IPR007864; UreE_C_dom.
DR InterPro; IPR004029; UreE_N.
DR InterPro; IPR036118; UreE_N_sf.
DR Pfam; PF05194; UreE_C; 1.
DR Pfam; PF02814; UreE_N; 1.
DR PIRSF; PIRSF036402; Ureas_acces_UreE; 1.
DR SMART; SM00988; UreE_N; 1.
DR SUPFAM; SSF69287; SSF69287; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chaperone; Cytoplasm; Nickel; Nickel insertion;
KW Reference proteome; Virulence.
FT CHAIN 1..170
FT /note="Urease accessory protein UreE"
FT /id="PRO_0000067633"
FT CONFLICT 7
FT /note="V -> I (in Ref. 1; AAA25023)"
FT /evidence="ECO:0000305"
FT CONFLICT 22
FT /note="H -> Y (in Ref. 1; AAA25023)"
FT /evidence="ECO:0000305"
FT CONFLICT 47
FT /note="I -> V (in Ref. 1; AAA25023)"
FT /evidence="ECO:0000305"
FT CONFLICT 57
FT /note="L -> F (in Ref. 1; AAA25023)"
FT /evidence="ECO:0000305"
FT CONFLICT 169
FT /note="V -> M (in Ref. 1; AAA25023)"
FT /evidence="ECO:0000305"
FT HELIX 10..12
FT /evidence="ECO:0007829|PDB:3TJ8"
FT HELIX 15..17
FT /evidence="ECO:0007829|PDB:3TJ8"
FT STRAND 18..25
FT /evidence="ECO:0007829|PDB:3TJ8"
FT HELIX 27..29
FT /evidence="ECO:0007829|PDB:3TJ8"
FT STRAND 33..38
FT /evidence="ECO:0007829|PDB:3TJ8"
FT STRAND 44..48
FT /evidence="ECO:0007829|PDB:3TJ8"
FT STRAND 61..66
FT /evidence="ECO:0007829|PDB:3TJ8"
FT STRAND 69..75
FT /evidence="ECO:0007829|PDB:3TJ8"
FT STRAND 78..84
FT /evidence="ECO:0007829|PDB:3TJ8"
FT HELIX 88..100
FT /evidence="ECO:0007829|PDB:3TJ8"
FT STRAND 105..107
FT /evidence="ECO:0007829|PDB:3TJ8"
FT STRAND 113..117
FT /evidence="ECO:0007829|PDB:3TJ8"
FT HELIX 120..129
FT /evidence="ECO:0007829|PDB:3TJ8"
FT STRAND 132..138
FT /evidence="ECO:0007829|PDB:3TJ8"
FT HELIX 142..144
FT /evidence="ECO:0007829|PDB:3TJ8"
FT STRAND 152..154
FT /evidence="ECO:0007829|PDB:3TJ9"
SQ SEQUENCE 170 AA; 19382 MW; 2B5FBBE3D0B21B3E CRC64;
MIIERLVGNL RDLNPLDFSV DHVDLEWFET RKKIARFKTR QGKDIAIRLK DAPKLGLSQG
DILFKEEKEI IAVNILDSEV IHIQAKSVAE VAKICYEIGN RHAALYYGES QFEFKTPFEK
PTLALLEKLG VQNRVLSSKL DSKERLTVSM PHSEPNFKVS LASDFKVVVK
