UREE_SPOPA
ID UREE_SPOPA Reviewed; 147 AA.
AC P50049; Q9RP20;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Urease accessory protein UreE;
GN Name=ureE;
OS Sporosarcina pasteurii (Bacillus pasteurii).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Planococcaceae; Sporosarcina.
OX NCBI_TaxID=1474;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 11859 / DSM 33 / NCIB 8841 / NCTC 4822;
RA You J.-H., Kim J.-G., Song B.-H., Lee M.-H., Kim S.-D.;
RT "Genetic organization and nucleotide sequence of the ure gene cluster in
RT Bacillus pasteurii.";
RL Mol. Cells 5:359-369(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], COFACTOR, SUBUNIT, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC STRAIN=ATCC 11859 / DSM 33 / NCIB 8841 / NCTC 4822;
RX PubMed=12072968; DOI=10.1007/s00775-002-0341-7;
RA Ciurli S., Safarov N., Miletti S., Dikiy A., Christensen S.K.,
RA Kornetzky K., Bryant D.A., Vandenberghe I., Devreese B., Samyn B.,
RA Remaut H., Van Beeumen J.;
RT "Molecular characterization of Bacillus pasteurii UreE, a metal-binding
RT chaperone for the assembly of the urease active site.";
RL J. Biol. Inorg. Chem. 7:623-631(2002).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
RX PubMed=11602602; DOI=10.1074/jbc.m108304200;
RA Remaut H., Safarov N., Ciurli S., Van Beeumen J.;
RT "Structural basis for Ni(2+) transport and assembly of the urease active
RT site by the metallochaperone UreE from Bacillus pasteurii.";
RL J. Biol. Chem. 276:49365-49370(2001).
CC -!- FUNCTION: Involved in urease metallocenter assembly. Binds nickel.
CC Probably functions as a nickel donor during metallocenter assembly.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000269|PubMed:12072968};
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC Evidence={ECO:0000269|PubMed:12072968};
CC Note=Binds 1 Zn(2+) or 1 Ni(2+) ion per dimer or tetramer.
CC {ECO:0000269|PubMed:12072968};
CC -!- SUBUNIT: Homodimer in diluted solutions, homotetramer in concentrated
CC solutions and when bound to Ni(2+) or Zn(2+). The homodimer is thought
CC to be the active form. {ECO:0000269|PubMed:12072968}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the UreE family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA73987.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U29368; AAA73987.1; ALT_INIT; Genomic_DNA.
DR EMBL; AF361945; AAD55059.1; -; Genomic_DNA.
DR PDB; 1EAR; X-ray; 1.70 A; A=3-147.
DR PDB; 1EB0; X-ray; 1.85 A; A=3-147.
DR PDB; 4L3K; X-ray; 1.88 A; A/B=1-147.
DR PDBsum; 1EAR; -.
DR PDBsum; 1EB0; -.
DR PDBsum; 4L3K; -.
DR AlphaFoldDB; P50049; -.
DR BMRB; P50049; -.
DR SMR; P50049; -.
DR EvolutionaryTrace; P50049; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051082; F:unfolded protein binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006457; P:protein folding; IEA:InterPro.
DR GO; GO:0065003; P:protein-containing complex assembly; IEA:InterPro.
DR GO; GO:0019627; P:urea metabolic process; IEA:InterPro.
DR CDD; cd00571; UreE; 1.
DR HAMAP; MF_00822; UreE; 1.
DR InterPro; IPR012406; UreE.
DR InterPro; IPR007864; UreE_C_dom.
DR InterPro; IPR004029; UreE_N.
DR InterPro; IPR036118; UreE_N_sf.
DR Pfam; PF05194; UreE_C; 1.
DR Pfam; PF02814; UreE_N; 1.
DR PIRSF; PIRSF036402; Ureas_acces_UreE; 1.
DR SMART; SM00988; UreE_N; 1.
DR SUPFAM; SSF69287; SSF69287; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chaperone; Cytoplasm; Metal-binding; Nickel;
KW Nickel insertion; Zinc.
FT CHAIN 1..147
FT /note="Urease accessory protein UreE"
FT /id="PRO_0000067629"
FT BINDING 100
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT HELIX 13..15
FT /evidence="ECO:0007829|PDB:1EAR"
FT STRAND 18..25
FT /evidence="ECO:0007829|PDB:1EAR"
FT HELIX 27..31
FT /evidence="ECO:0007829|PDB:1EAR"
FT STRAND 33..38
FT /evidence="ECO:0007829|PDB:1EAR"
FT STRAND 44..48
FT /evidence="ECO:0007829|PDB:1EAR"
FT STRAND 50..52
FT /evidence="ECO:0007829|PDB:4L3K"
FT STRAND 59..63
FT /evidence="ECO:0007829|PDB:1EAR"
FT STRAND 65..73
FT /evidence="ECO:0007829|PDB:1EAR"
FT STRAND 76..82
FT /evidence="ECO:0007829|PDB:1EAR"
FT HELIX 86..98
FT /evidence="ECO:0007829|PDB:1EAR"
FT STRAND 104..106
FT /evidence="ECO:0007829|PDB:1EAR"
FT STRAND 109..113
FT /evidence="ECO:0007829|PDB:1EAR"
FT HELIX 116..125
FT /evidence="ECO:0007829|PDB:1EAR"
FT STRAND 128..134
FT /evidence="ECO:0007829|PDB:1EAR"
SQ SEQUENCE 147 AA; 17402 MW; 8E07578B67230DD3 CRC64;
MLITKIVGHI DDYESSDKKV DWLEVEWEDL NKRILRKETE NGTDIAIKLE NSGTLRYGDV
LYESDDTLIA IRTKLEKVYV IKPQTMQEMG KMAFEIGNRH TMCIIEDDEI LVRYDKTLEK
LIDEVGVSYE QSERRFKEPF KYRGHQH
