ID   UREF1_BRUA2             Reviewed;         228 AA.
AC   Q2YPD3; Q57F83; Q93T79;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Urease accessory protein UreF 1 {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF1 {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=BAB1_0302;
OS   Brucella abortus (strain 2308).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=359391;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND ROLE IN VIRULENCE.
RX   PubMed=17101645; DOI=10.1128/iai.01244-06;
RA   Sangari F.J., Seoane A., Rodriguez M.C., Aguero J., Garcia Lobo J.M.;
RT   "Characterization of the urease operon of Brucella abortus and assessment
RT   of its role in virulence of the bacterium.";
RL   Infect. Immun. 75:774-780(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=2308;
RX   PubMed=16299333; DOI=10.1128/iai.73.12.8353-8361.2005;
RA   Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A.,
RA   Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.;
RT   "Whole-genome analyses of speciation events in pathogenic Brucellae.";
RL   Infect. Immun. 73:8353-8361(2005).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- FUNCTION: Disruption of the ure1 gene cluster suggests that it protects
CC       brucellae during their passage through the stomach. The major route of
CC       infection in human brucellosis is oral. {ECO:0000269|PubMed:17101645}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; AF361941; AAK51071.1; -; Genomic_DNA.
DR   EMBL; AM040264; CAJ10258.1; -; Genomic_DNA.
DR   RefSeq; WP_002969031.1; NZ_KN046823.1.
DR   AlphaFoldDB; Q2YPD3; -.
DR   SMR; Q2YPD3; -.
DR   STRING; 359391.BAB1_0302; -.
DR   EnsemblBacteria; CAJ10258; CAJ10258; BAB1_0302.
DR   GeneID; 3787080; -.
DR   KEGG; bmf:BAB1_0302; -.
DR   PATRIC; fig|359391.11.peg.2350; -.
DR   HOGENOM; CLU_049215_2_0_5; -.
DR   OMA; TQYTRLF; -.
DR   Proteomes; UP000002719; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion; Reference proteome; Virulence.
FT   CHAIN           1..228
FT                   /note="Urease accessory protein UreF 1"
FT                   /id="PRO_0000344085"
SQ   SEQUENCE   228 AA;  24450 MW;  019E0C3CE1314F19 CRC64;
     MTIITPITND PTTALLRLMA WLSPVFPVGS FSYSHGLERA VHDGLVVDAA GLQDWLQWLV
     RRGSGWNDAV LCAESWRCAM KGEDLHEIAE LAEALAGSRE RHMETMLQGG AFFAAARSWP
     CEIFDRLPPD CAYPVAVGAV AGGHGVPLAQ ALAAFLQAFC INLLQASIRL SVTGQSGVTA
     IMAALEPVLG ETAARAALSS MEDLGSATFI ADIMAMKHET QHSRLFRS