ID   UREF1_BRUME             Reviewed;         241 AA.
AC   Q8YI00;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Urease accessory protein UreF 1 {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF1 {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=BMEI0645;
OS   Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=224914;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=16M / ATCC 23456 / NCTC 10094;
RX   PubMed=11756688; DOI=10.1073/pnas.221575398;
RA   DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T.,
RA   Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G.,
RA   Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E.,
RA   Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J.,
RA   Haselkorn R., Kyrpides N.C., Overbeek R.;
RT   "The genome sequence of the facultative intracellular pathogen Brucella
RT   melitensis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; AE008917; AAL51826.1; -; Genomic_DNA.
DR   PIR; AG3332; AG3332.
DR   AlphaFoldDB; Q8YI00; -.
DR   SMR; Q8YI00; -.
DR   STRING; 224914.BMEI0645; -.
DR   EnsemblBacteria; AAL51826; AAL51826; BMEI0645.
DR   KEGG; bme:BMEI0645; -.
DR   eggNOG; COG0830; Bacteria.
DR   OMA; HVRLFMN; -.
DR   PhylomeDB; Q8YI00; -.
DR   Proteomes; UP000000419; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion.
FT   CHAIN           1..241
FT                   /note="Urease accessory protein UreF 1"
FT                   /id="PRO_0000344090"
SQ   SEQUENCE   241 AA;  26332 MW;  6E1AD9DDC33F7F6C CRC64;
     MRTATITEFS SSYRSLPGLL HLLQFGDSAL PIGGFSFSNG LESAIQQNLV HDKETLREFT
     LTAMNQAATS DRIALLTAHR AARADDRGAL QVIDKAVFER KLNEETRLMT VRMGRKLCEL
     SASIIDDRLN RDWLECIKTA ETPGTHPVSL GLAFAALDVD GRDAFGAQQY GVATTILGAA
     LRLMRVSFMD TQKILLEATS TVAPAYEEIA DAGIEDMASF APMVDILAAV HVKGHVRMFM
     N