ID   UREF1_BRUSI             Reviewed;         228 AA.
AC   B0CJP9;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 1.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=Urease accessory protein UreF 1 {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF1 {ECO:0000255|HAMAP-Rule:MF_01385};
GN   OrderedLocusNames=BSUIS_A0295;
OS   Brucella suis (strain ATCC 23445 / NCTC 10510).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=470137;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 23445 / NCTC 10510;
RA   Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C.,
RA   Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S.,
RA   Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M.,
RA   Snyder E.E., Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H.,
RA   Bruce D., Detter C., Munk C., Brettin T.S.;
RT   "Brucella suis ATCC 23445 whole genome shotgun sequencing project.";
RL   Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; CP000911; ABY37390.1; -; Genomic_DNA.
DR   RefSeq; WP_004690532.1; NC_010169.1.
DR   AlphaFoldDB; B0CJP9; -.
DR   SMR; B0CJP9; -.
DR   EnsemblBacteria; ABY37390; ABY37390; BSUIS_A0295.
DR   GeneID; 45051405; -.
DR   GeneID; 55590051; -.
DR   KEGG; bmt:BSUIS_A0295; -.
DR   HOGENOM; CLU_049215_2_0_5; -.
DR   OMA; TQYTRLF; -.
DR   Proteomes; UP000008545; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion.
FT   CHAIN           1..228
FT                   /note="Urease accessory protein UreF 1"
FT                   /id="PRO_0000344094"
SQ   SEQUENCE   228 AA;  24428 MW;  B15704672DDFC361 CRC64;
     MIIITPITND PTTALLRLMA WLSPVFPVGS FSYSHGLERA VHDGLVVDAA GLQDWLQWLV
     RRGSGWNDAV LCAESWRCAM KGEDLHEIAE LAEALAGSRE RHMETMLQGG AFLAAARSWP
     CEIFDRLPPD CAYPVAVGAV AGGHGVPLAQ ALAAFLQAFC INLLQASIRL SVTGQSGVTA
     IMAALEPVLG ETAARAALSS MEDLGSATFI ADIMAMKHET QHSRLFRS