ID   UREF1_BRUSU             Reviewed;         228 AA.
AC   Q8G2P6; G0KBX1;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Urease accessory protein UreF 1 {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF1 {ECO:0000255|HAMAP-Rule:MF_01385};
GN   OrderedLocusNames=BR0272, BS1330_I0273;
OS   Brucella suis biovar 1 (strain 1330).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=204722;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=12271122; DOI=10.1073/pnas.192319099;
RA   Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F.,
RA   Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA   Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J.A., Van Aken S.E.,
RA   Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L.,
RA   Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.;
RT   "The Brucella suis genome reveals fundamental similarities between animal
RT   and plant pathogens and symbionts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=22038969; DOI=10.1128/jb.06181-11;
RA   Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.;
RT   "Revised genome sequence of Brucella suis 1330.";
RL   J. Bacteriol. 193:6410-6410(2011).
RN   [3]
RP   OPERON DISRUPTION, AND ROLE IN VIRULENCE.
RC   STRAIN=1330;
RX   PubMed=17578575; DOI=10.1186/1471-2180-7-57;
RA   Bandara A.B., Contreras A., Contreras-Rodriguez A., Martins A.M.,
RA   Dobrean V., Poff-Reichow S., Rajasekaran P., Sriranganathan N.,
RA   Schurig G.G., Boyle S.M.;
RT   "Brucella suis urease encoded by ure1 but not ure2 is necessary for
RT   intestinal infection of BALB/c mice.";
RL   BMC Microbiol. 7:57-57(2007).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- FUNCTION: Disrupting the ure1 operon causes loss of urease activity,
CC       decreased resistance to low pH killing in vitro and decreased pathogen
CC       survival when inoculated in BALB/c mice by gavage.
CC       {ECO:0000269|PubMed:17578575}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; AE014291; AAN29221.1; -; Genomic_DNA.
DR   EMBL; CP002997; AEM17634.1; -; Genomic_DNA.
DR   RefSeq; WP_004690532.1; NZ_KN046804.1.
DR   AlphaFoldDB; Q8G2P6; -.
DR   SMR; Q8G2P6; -.
DR   EnsemblBacteria; AEM17634; AEM17634; BS1330_I0273.
DR   GeneID; 45051405; -.
DR   GeneID; 55590051; -.
DR   KEGG; bms:BR0272; -.
DR   KEGG; bsi:BS1330_I0273; -.
DR   PATRIC; fig|204722.21.peg.1755; -.
DR   HOGENOM; CLU_049215_2_0_5; -.
DR   OMA; TQYTRLF; -.
DR   PhylomeDB; Q8G2P6; -.
DR   Proteomes; UP000007104; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion; Virulence.
FT   CHAIN           1..228
FT                   /note="Urease accessory protein UreF 1"
FT                   /id="PRO_0000344092"
SQ   SEQUENCE   228 AA;  24428 MW;  B15704672DDFC361 CRC64;
     MIIITPITND PTTALLRLMA WLSPVFPVGS FSYSHGLERA VHDGLVVDAA GLQDWLQWLV
     RRGSGWNDAV LCAESWRCAM KGEDLHEIAE LAEALAGSRE RHMETMLQGG AFLAAARSWP
     CEIFDRLPPD CAYPVAVGAV AGGHGVPLAQ ALAAFLQAFC INLLQASIRL SVTGQSGVTA
     IMAALEPVLG ETAARAALSS MEDLGSATFI ADIMAMKHET QHSRLFRS