ID   UREF1_PSESM             Reviewed;         243 AA.
AC   Q883F2;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Urease accessory protein UreF 1 {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF1 {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=PSPTO_2412;
OS   Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=223283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-871 / DC3000;
RX   PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA   Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA   Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA   Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA   Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA   Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA   Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA   Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA   Collmer A.;
RT   "The complete genome sequence of the Arabidopsis and tomato pathogen
RT   Pseudomonas syringae pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; AE016853; AAO55921.1; -; Genomic_DNA.
DR   RefSeq; NP_792226.1; NC_004578.1.
DR   RefSeq; WP_011103990.1; NC_004578.1.
DR   AlphaFoldDB; Q883F2; -.
DR   SMR; Q883F2; -.
DR   STRING; 223283.PSPTO_2412; -.
DR   DNASU; 1184064; -.
DR   EnsemblBacteria; AAO55921; AAO55921; PSPTO_2412.
DR   GeneID; 1184064; -.
DR   KEGG; pst:PSPTO_2412; -.
DR   PATRIC; fig|223283.9.peg.2449; -.
DR   eggNOG; COG0830; Bacteria.
DR   HOGENOM; CLU_049215_4_0_6; -.
DR   OMA; FRWAETD; -.
DR   OrthoDB; 1942614at2; -.
DR   PhylomeDB; Q883F2; -.
DR   Proteomes; UP000002515; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion; Reference proteome.
FT   CHAIN           1..243
FT                   /note="Urease accessory protein UreF 1"
FT                   /id="PRO_0000344155"
SQ   SEQUENCE   243 AA;  26366 MW;  659D8A6C3CA9970C CRC64;
     MNTLSSLLLA LQQADSFFPG GAVAWSWGLE TLVADGRLGA GERVTARRRQ RAVRLDRSAA
     VRGFVEGQLR HRWHSFDRVF LLAAWNAADD VSALTNLDAQ IEALTLAKEL RQGSRRVGQA
     LLGVHVALGT PGAAAYQQQV RAGNTPGHLP VLQGLLWNRL GMHREHCQLA AAHGLCTGLV
     SAAVRLGVMG HIDAQRVLTD IQPLITELLA LEPPEPEDAC GFTPMAEIAV MRHETQDLRL
     FAN