ID   UREF2_BRUAB             Reviewed;         243 AA.
AC   Q57CE6;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Urease accessory protein UreF 2 {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF2 {ECO:0000255|HAMAP-Rule:MF_01385};
GN   OrderedLocusNames=BruAb1_1357;
OS   Brucella abortus biovar 1 (strain 9-941).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=262698;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=9-941;
RX   PubMed=15805518; DOI=10.1128/jb.187.8.2715-2726.2005;
RA   Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z.,
RA   Li L.-L., Kapur V., Alt D.P., Olsen S.C.;
RT   "Completion of the genome sequence of Brucella abortus and comparison to
RT   the highly similar genomes of Brucella melitensis and Brucella suis.";
RL   J. Bacteriol. 187:2715-2726(2005).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; AE017223; AAX74688.1; -; Genomic_DNA.
DR   RefSeq; WP_002966883.1; NC_006932.1.
DR   AlphaFoldDB; Q57CE6; -.
DR   SMR; Q57CE6; -.
DR   EnsemblBacteria; AAX74688; AAX74688; BruAb1_1357.
DR   GeneID; 45052380; -.
DR   GeneID; 55591020; -.
DR   KEGG; bmb:BruAb1_1357; -.
DR   HOGENOM; CLU_049215_4_0_5; -.
DR   OMA; HVRLFMN; -.
DR   Proteomes; UP000000540; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion.
FT   CHAIN           1..243
FT                   /note="Urease accessory protein UreF 2"
FT                   /id="PRO_0000344084"
SQ   SEQUENCE   243 AA;  26465 MW;  CE0A0602F0527ECB CRC64;
     MTMRTATITE FSSSYRSLPG LLHLLQFGDS ALPIGGFSFS NGLESAIQQN LVHDKETLRE
     FTLTAMNQAA TSDGIALLTA HRAARADDRG ALQVIDKAVF ERKLNEETRL MTVRMGRKLC
     ELSASIIDDR LNRDWLECIK TAETPGTHPV SLGLAFAALD VDGRDAFGAQ QYGVATTILG
     AALRLMRVSF MDTQKILLEA TSTVAPAYEE IADAGIEDMA SFAPMVDILA AVHVKGHVRM
     FMN