ID   UREF2_BRUME             Reviewed;         228 AA.
AC   Q8YF73;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Urease accessory protein UreF 2 {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF2 {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=BMEI1650;
OS   Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=224914;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=16M / ATCC 23456 / NCTC 10094;
RX   PubMed=11756688; DOI=10.1073/pnas.221575398;
RA   DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T.,
RA   Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G.,
RA   Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E.,
RA   Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J.,
RA   Haselkorn R., Kyrpides N.C., Overbeek R.;
RT   "The genome sequence of the facultative intracellular pathogen Brucella
RT   melitensis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; AE008917; AAL52831.1; -; Genomic_DNA.
DR   PIR; AD3458; AD3458.
DR   RefSeq; WP_004682815.1; NZ_GG703778.1.
DR   AlphaFoldDB; Q8YF73; -.
DR   SMR; Q8YF73; -.
DR   STRING; 224914.BMEI1650; -.
DR   EnsemblBacteria; AAL52831; AAL52831; BMEI1650.
DR   GeneID; 29594503; -.
DR   KEGG; bme:BMEI1650; -.
DR   KEGG; bmel:DK63_1840; -.
DR   PATRIC; fig|224914.52.peg.1941; -.
DR   eggNOG; COG0830; Bacteria.
DR   OMA; TQYTRLF; -.
DR   Proteomes; UP000000419; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion.
FT   CHAIN           1..228
FT                   /note="Urease accessory protein UreF 2"
FT                   /id="PRO_0000344089"
SQ   SEQUENCE   228 AA;  24446 MW;  F0D10C2F06ADC49A CRC64;
     MTIITPITND PTTALLRLMA WLSPVFPVGS FSYSHGLERA VHDGLVVDAA GLQDWLQWLV
     RRGSGWNDAV LCAESWRCTM KGEDLHEIAE LAEALAGSRE RHMETMLQGG AFLAAARSWP
     CEIFDRLPPD CAYPVAVGAV AGGHGVPLAQ ALAAFLQAFC INLLQASIRL SVTGQSGVTA
     IMAALEPVLG ETAARAALSS MEDLGSATFI ADIMAMKHET QHSRLFRS