ID   UREF2_BRUSU             Reviewed;         243 AA.
AC   Q8FZW0; G0KB45;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Urease accessory protein UreF 2 {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF2 {ECO:0000255|HAMAP-Rule:MF_01385};
GN   OrderedLocusNames=BR1360, BS1330_I1355;
OS   Brucella suis biovar 1 (strain 1330).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=204722;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=12271122; DOI=10.1073/pnas.192319099;
RA   Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F.,
RA   Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA   Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J.A., Van Aken S.E.,
RA   Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L.,
RA   Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.;
RT   "The Brucella suis genome reveals fundamental similarities between animal
RT   and plant pathogens and symbionts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=22038969; DOI=10.1128/jb.06181-11;
RA   Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.;
RT   "Revised genome sequence of Brucella suis 1330.";
RL   J. Bacteriol. 193:6410-6410(2011).
RN   [3]
RP   OPERON DISRUPTION, AND LACK OF ROLE IN VIRULENCE.
RC   STRAIN=1330;
RX   PubMed=17578575; DOI=10.1186/1471-2180-7-57;
RA   Bandara A.B., Contreras A., Contreras-Rodriguez A., Martins A.M.,
RA   Dobrean V., Poff-Reichow S., Rajasekaran P., Sriranganathan N.,
RA   Schurig G.G., Boyle S.M.;
RT   "Brucella suis urease encoded by ure1 but not ure2 is necessary for
RT   intestinal infection of BALB/c mice.";
RL   BMC Microbiol. 7:57-57(2007).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- FUNCTION: Disrupting the ure2 operon has no effect on urease activity,
CC       or pathogen survival in BALB/c mice when inoculated by gavage, but
CC       confers slightly enhanced resistance to low pH killing in vitro.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; AE014291; AAN30274.1; -; Genomic_DNA.
DR   EMBL; CP002997; AEM18691.1; -; Genomic_DNA.
DR   RefSeq; WP_002966883.1; NZ_KN046804.1.
DR   AlphaFoldDB; Q8FZW0; -.
DR   SMR; Q8FZW0; -.
DR   EnsemblBacteria; AEM18691; AEM18691; BS1330_I1355.
DR   GeneID; 45052380; -.
DR   GeneID; 55591020; -.
DR   KEGG; bms:BR1360; -.
DR   KEGG; bsi:BS1330_I1355; -.
DR   PATRIC; fig|204722.21.peg.835; -.
DR   HOGENOM; CLU_049215_4_0_5; -.
DR   OMA; HVRLFMN; -.
DR   PhylomeDB; Q8FZW0; -.
DR   Proteomes; UP000007104; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion.
FT   CHAIN           1..243
FT                   /note="Urease accessory protein UreF 2"
FT                   /id="PRO_0000344093"
SQ   SEQUENCE   243 AA;  26465 MW;  CE0A0602F0527ECB CRC64;
     MTMRTATITE FSSSYRSLPG LLHLLQFGDS ALPIGGFSFS NGLESAIQQN LVHDKETLRE
     FTLTAMNQAA TSDGIALLTA HRAARADDRG ALQVIDKAVF ERKLNEETRL MTVRMGRKLC
     ELSASIIDDR LNRDWLECIK TAETPGTHPV SLGLAFAALD VDGRDAFGAQ QYGVATTILG
     AALRLMRVSF MDTQKILLEA TSTVAPAYEE IADAGIEDMA SFAPMVDILA AVHVKGHVRM
     FMN