ID   UREF2_PSESM             Reviewed;         224 AA.
AC   Q87VM6;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Urease accessory protein UreF 2 {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF2 {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=PSPTO_4910;
OS   Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=223283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-871 / DC3000;
RX   PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA   Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA   Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA   Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA   Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA   Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA   Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA   Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA   Collmer A.;
RT   "The complete genome sequence of the Arabidopsis and tomato pathogen
RT   Pseudomonas syringae pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; AE016853; AAO58338.1; -; Genomic_DNA.
DR   RefSeq; NP_794643.1; NC_004578.1.
DR   RefSeq; WP_005763065.1; NC_004578.1.
DR   AlphaFoldDB; Q87VM6; -.
DR   SMR; Q87VM6; -.
DR   STRING; 223283.PSPTO_4910; -.
DR   PRIDE; Q87VM6; -.
DR   DNASU; 1186593; -.
DR   EnsemblBacteria; AAO58338; AAO58338; PSPTO_4910.
DR   GeneID; 1186593; -.
DR   KEGG; pst:PSPTO_4910; -.
DR   PATRIC; fig|223283.9.peg.5023; -.
DR   eggNOG; COG0830; Bacteria.
DR   HOGENOM; CLU_049215_2_1_6; -.
DR   OMA; TQYTRLF; -.
DR   OrthoDB; 1942614at2; -.
DR   PhylomeDB; Q87VM6; -.
DR   Proteomes; UP000002515; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion; Reference proteome.
FT   CHAIN           1..224
FT                   /note="Urease accessory protein UreF 2"
FT                   /id="PRO_0000344156"
SQ   SEQUENCE   224 AA;  24939 MW;  75A4FC0343251E24 CRC64;
     MNSAWALLRL ASPQLPIGGY SYSQGLEMAV EQSIVIDPQT AGRWIGDQLL LNLARFEAPL
     LLAHCEAAAV GDWGQLLQVS EQHRASRETR ELHQESRQMG YSLQQLLNGL PELDRDARRF
     LEQTTEPHLA LGWALAARTW RISPQDALAA WLWSWLENQL AVLMKTLPLG QQAAQRLTSE
     LLPLLQQAQV NASAQDTHPA GSAAFGLALA SMAHERQYSR LFRS