ID   UREF_ACTP2              Reviewed;         227 AA.
AC   A3N2R1;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2007, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=APL_1613;
OS   Actinobacillus pleuropneumoniae serotype 5b (strain L20).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Actinobacillus.
OX   NCBI_TaxID=416269;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=L20;
RX   PubMed=18065534; DOI=10.1128/jb.01845-07;
RA   Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M.,
RA   Nash J.H.E.;
RT   "The complete genome sequence of Actinobacillus pleuropneumoniae L20
RT   (serotype 5b).";
RL   J. Bacteriol. 190:1495-1496(2008).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; CP000569; ABN74697.1; -; Genomic_DNA.
DR   AlphaFoldDB; A3N2R1; -.
DR   SMR; A3N2R1; -.
DR   STRING; 416269.APL_1613; -.
DR   EnsemblBacteria; ABN74697; ABN74697; APL_1613.
DR   KEGG; apl:APL_1613; -.
DR   eggNOG; COG0830; Bacteria.
DR   HOGENOM; CLU_049215_4_2_6; -.
DR   OMA; TQYTRLF; -.
DR   Proteomes; UP000001432; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion; Reference proteome.
FT   CHAIN           1..227
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_1000145107"
SQ   SEQUENCE   227 AA;  25468 MW;  36243EFADE479D22 CRC64;
     MGQLGALLHL VDPTLPIGGF NHSNGLETFV QQGKVNSRAS LEEYVQTQLM QNWIYNDGAY
     LSLAFDAMAN HDLDRLLQLD QELAASKIAR ESREGSYKLG VRLLKIFIRY ENHPLLSEFQ
     QAISEKRCQG YFPIVFAMVA QAMNLDKAET LYAFYYNAAV GVVTNGVKLV PLSQMDGQDI
     LFALRTPLAQ AVENSLNPDL DWLGAATLAS DIRSMQHEQL YTRLYMS