ID   UREF_ALIF1              Reviewed;         221 AA.
AC   Q5E730;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=VF_0671;
OS   Aliivibrio fischeri (strain ATCC 700601 / ES114) (Vibrio fischeri).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Aliivibrio.
OX   NCBI_TaxID=312309;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700601 / ES114;
RX   PubMed=15703294; DOI=10.1073/pnas.0409900102;
RA   Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R.,
RA   Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E.,
RA   Stevens A., Visick K., Whistler C., Greenberg E.P.;
RT   "Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with
RT   pathogenic congeners.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; CP000020; AAW85166.1; -; Genomic_DNA.
DR   RefSeq; WP_005418029.1; NC_006840.2.
DR   RefSeq; YP_204054.1; NC_006840.2.
DR   AlphaFoldDB; Q5E730; -.
DR   SMR; Q5E730; -.
DR   STRING; 312309.VF_0671; -.
DR   DNASU; 3277360; -.
DR   EnsemblBacteria; AAW85166; AAW85166; VF_0671.
DR   GeneID; 64242915; -.
DR   KEGG; vfi:VF_0671; -.
DR   PATRIC; fig|312309.11.peg.664; -.
DR   eggNOG; COG0830; Bacteria.
DR   HOGENOM; CLU_049215_2_1_6; -.
DR   OMA; TQYTRLF; -.
DR   OrthoDB; 1942614at2; -.
DR   Proteomes; UP000000537; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion; Reference proteome.
FT   CHAIN           1..221
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_0000344200"
SQ   SEQUENCE   221 AA;  25059 MW;  6E634F78B43496D7 CRC64;
     MLEDLRLYQL ISPSLPVGSF TYSQGLEWAI EKGWVTNVTE LKHWLSNQLM DSLATLELPV
     LAKLTQLLQQ EEWQQAQDWC DFIIANRETK ELRLEERQRG LAFSMLLPKL GIELNQTTLP
     MVKQTQVAAF ALAANHWNLT PTKLAAAYAW GWLENAVIVG IKLVPLGQSA GQQLLLEMAD
     VIPQAVEKSQ HWPEHLIGSF TPAQVLASSR HESQYTRLFR S