ID   UREF_ARATH              Reviewed;         240 AA.
AC   Q9XHZ3; E0ZS50;
DT   13-NOV-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Urease accessory protein F;
DE            Short=AtUREF;
GN   Name=UREF; OrderedLocusNames=At1g21840; ORFNames=F8K7.27, T26F17.5;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Shinn P., Chen H., Cheuk R.F., Kim C.J., Carninci P., Hayashizaki Y.,
RA   Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA   Shinozaki K., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-86.
RC   STRAIN=cv. Columbia;
RX   PubMed=20631318; DOI=10.1104/pp.110.160929;
RA   Cao F.Q., Werner A.K., Dahncke K., Romeis T., Liu L.H., Witte C.P.;
RT   "Identification and characterization of proteins involved in rice urea and
RT   arginine catabolism.";
RL   Plant Physiol. 154:98-108(2010).
RN   [6]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=16244137; DOI=10.1104/pp.105.070292;
RA   Witte C.P., Rosso M.G., Romeis T.;
RT   "Identification of three urease accessory proteins that are required for
RT   urease activation in Arabidopsis.";
RL   Plant Physiol. 139:1155-1162(2005).
CC   -!- FUNCTION: Required for the maturation and activation of urease via the
CC       functional incorporation of the urease nickel metallocenter.
CC       {ECO:0000269|PubMed:16244137}.
CC   -!- SUBUNIT: URED, UREF and UREG may form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein. {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC       conditions, but mutant plants cannot grow on medium with urea as the
CC       sole source of nitrogen. {ECO:0000269|PubMed:16244137}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000305}.
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DR   EMBL; AC007727; AAD41438.1; -; Genomic_DNA.
DR   EMBL; AC013482; AAF16528.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE30161.1; -; Genomic_DNA.
DR   EMBL; CP002684; ANM60497.1; -; Genomic_DNA.
DR   EMBL; BT012628; AAT06447.1; -; mRNA.
DR   EMBL; AK175196; BAD42959.1; -; mRNA.
DR   EMBL; HM369062; ADK74001.1; -; mRNA.
DR   RefSeq; NP_001322780.1; NM_001332530.1.
DR   RefSeq; NP_173602.1; NM_102032.4.
DR   AlphaFoldDB; Q9XHZ3; -.
DR   SMR; Q9XHZ3; -.
DR   BioGRID; 24025; 3.
DR   ComplexPortal; CPX-1296; Urease activation complex.
DR   IntAct; Q9XHZ3; 3.
DR   STRING; 3702.AT1G21840.1; -.
DR   PaxDb; Q9XHZ3; -.
DR   PRIDE; Q9XHZ3; -.
DR   ProteomicsDB; 228628; -.
DR   DNASU; 838786; -.
DR   EnsemblPlants; AT1G21840.1; AT1G21840.1; AT1G21840.
DR   EnsemblPlants; AT1G21840.2; AT1G21840.2; AT1G21840.
DR   GeneID; 838786; -.
DR   Gramene; AT1G21840.1; AT1G21840.1; AT1G21840.
DR   Gramene; AT1G21840.2; AT1G21840.2; AT1G21840.
DR   KEGG; ath:AT1G21840; -.
DR   Araport; AT1G21840; -.
DR   TAIR; locus:2036997; AT1G21840.
DR   eggNOG; ENOG502REVQ; Eukaryota.
DR   HOGENOM; CLU_049215_1_0_1; -.
DR   InParanoid; Q9XHZ3; -.
DR   OMA; VMDLWIG; -.
DR   OrthoDB; 1462110at2759; -.
DR   PhylomeDB; Q9XHZ3; -.
DR   PRO; PR:Q9XHZ3; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9XHZ3; baseline and differential.
DR   Genevisible; Q9XHZ3; AT.
DR   GO; GO:0150006; C:urease activator complex; IPI:ComplexPortal.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IMP:TAIR.
DR   GO; GO:1905182; P:positive regulation of urease activity; IMP:TAIR.
DR   GO; GO:0043419; P:urea catabolic process; IDA:ComplexPortal.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   2: Evidence at transcript level;
KW   Chaperone; Nickel insertion; Reference proteome.
FT   CHAIN           1..240
FT                   /note="Urease accessory protein F"
FT                   /id="PRO_0000424252"
SQ   SEQUENCE   240 AA;  26403 MW;  BF4437883876ED38 CRC64;
     MEEDERRDIV MSRASSCMQW SQWQLLDSIL PTGGFAHSFG LEAAIQTRLV SSPEDLETHI
     IHVLDNTASL LLPFVYSALK SPDIETWHKL DGILNATLTN QVSSKASMSQ GSALFRIAAS
     VFTEVPNLKM IRDASLGSKN VCFHHAPIFG LVCGLLGMDS ETSQRAYLFV TLRDVLSAAT
     RLNIVGPMGA SVMQHRIAIV TETVLEKWMN REAGEACQTS PLLDVVQGCH GYLFSRLFCS