ID   UREF_BACSB              Reviewed;         227 AA.
AC   Q07402;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   25-MAY-2022, entry version 50.
DE   RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385};
OS   Bacillus sp. (strain TB-90).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=36824;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8288539; DOI=10.1128/jb.176.2.432-442.1994;
RA   Maeda M., Hidaka M., Nakamura A., Masaki H., Uozumi T.;
RT   "Cloning, sequencing, and expression of thermophilic Bacillus sp. strain
RT   TB-90 urease gene complex in Escherichia coli.";
RL   J. Bacteriol. 176:432-442(1994).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; D14439; BAA03327.1; -; Genomic_DNA.
DR   PIR; E36950; E36950.
DR   AlphaFoldDB; Q07402; -.
DR   SMR; Q07402; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion.
FT   CHAIN           1..227
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_0000067645"
SQ   SEQUENCE   227 AA;  25447 MW;  4515BB29B186D60B CRC64;
     MNRLLSLFQL CDSNFPSGSF SHSFGLETYI QEKVITDKES FKNAISVYIR KQLFFTEGLA
     CILAYEAMEK NEPSALVELD HILFASNVAQ ETRSGNQRMG ERMAKLCVDL YPSPILIEYT
     NRIKEKKAYG HSAIVFAIVA YHLKVTKETA VGAYLFANVS ALVQNAVRGI PIGQTDGQRI
     LVEIQPLLEE GVRTISQLPK EDLGAVSPGM EIAQMRHERL NVRLFMS