UREF_CORGL
ID UREF_CORGL Reviewed; 226 AA.
AC Q79VJ2; Q9L418; Q9RHM2;
DT 22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385};
GN OrderedLocusNames=Cgl0088, cg0117;
OS Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 /
OS JCM 1318 / LMG 3730 / NCIMB 10025).
OC Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
OC Corynebacterium.
OX NCBI_TaxID=196627;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC STRAIN=ATCC 13869 / DSMZ 1412 / NCIMB 9567;
RX PubMed=11328647; DOI=10.3109/10425170009033989;
RA Puskas L.G., Inui M., Yukawa H.;
RT "Structure of the urease operon of Corynebacterium glutamicum.";
RL DNA Seq. 11:383-394(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROBABLE OPERON STRUCTURE.
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=10930756; DOI=10.1111/j.1574-6968.2000.tb09248.x;
RA Nolden L., Beckers G., Moeckel B., Pfefferle W., Nampoothiri K.M.,
RA Kraemer R., Burkovski A.;
RT "Urease of Corynebacterium glutamicum: organization of corresponding genes
RT and investigation of activity.";
RL FEMS Microbiol. Lett. 189:305-310(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=12743753; DOI=10.1007/s00253-003-1328-1;
RA Ikeda M., Nakagawa S.;
RT "The Corynebacterium glutamicum genome: features and impacts on
RT biotechnological processes.";
RL Appl. Microbiol. Biotechnol. 62:99-109(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=12948626; DOI=10.1016/s0168-1656(03)00154-8;
RA Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A.,
RA Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A.,
RA Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F.,
RA Moeckel B., Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O.,
RA Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.;
RT "The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its
RT impact on the production of L-aspartate-derived amino acids and vitamins.";
RL J. Biotechnol. 104:5-25(2003).
CC -!- FUNCTION: Required for maturation of urease via the functional
CC incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC Rule:MF_01385}.
CC -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC hydrolysis-dependent molecular chaperone, activating the urease
CC apoprotein by helping to assemble the nickel containing metallocenter
CC of UreC. The UreE protein probably delivers the nickel.
CC {ECO:0000255|HAMAP-Rule:MF_01385}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC -!- INDUCTION: By urea and nitrogen starvation.
CC {ECO:0000269|PubMed:11328647}.
CC -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC Rule:MF_01385}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA88556.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB029154; BAA88556.1; ALT_INIT; Genomic_DNA.
DR EMBL; AJ251883; CAB81939.1; -; Genomic_DNA.
DR EMBL; BA000036; BAB97481.1; -; Genomic_DNA.
DR EMBL; BX927148; CAF18656.1; -; Genomic_DNA.
DR RefSeq; NP_599340.1; NC_003450.3.
DR RefSeq; WP_011013380.1; NC_006958.1.
DR AlphaFoldDB; Q79VJ2; -.
DR SMR; Q79VJ2; -.
DR STRING; 196627.cg0117; -.
DR DNASU; 3342891; -.
DR KEGG; cgb:cg0117; -.
DR KEGG; cgl:Cgl0088; -.
DR PATRIC; fig|196627.13.peg.89; -.
DR eggNOG; COG0830; Bacteria.
DR HOGENOM; CLU_049215_4_2_11; -.
DR OMA; TQYTRLF; -.
DR Proteomes; UP000000582; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR Gene3D; 1.10.4190.10; -; 1.
DR HAMAP; MF_01385; UreF; 1.
DR InterPro; IPR002639; UreF.
DR InterPro; IPR038277; UreF_sf.
DR Pfam; PF01730; UreF; 1.
DR PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE 2: Evidence at transcript level;
KW Chaperone; Cytoplasm; Nickel insertion; Reference proteome.
FT CHAIN 1..226
FT /note="Urease accessory protein UreF"
FT /id="PRO_0000344116"
FT CONFLICT 2..22
FT /note="DLDADFLLLHLSDSALPTGAF -> MISTLISAVALSGSATSTGRV (in
FT Ref. 1; BAA88556)"
FT /evidence="ECO:0000305"
FT CONFLICT 26..30
FT /note="FGFET -> LGLKN (in Ref. 1; BAA88556)"
FT /evidence="ECO:0000305"
FT CONFLICT 39..46
FT /note="NAEEFQDW -> MQRSSKTG (in Ref. 1; BAA88556)"
FT /evidence="ECO:0000305"
FT CONFLICT 179
FT /note="V -> M (in Ref. 1; BAA88556)"
FT /evidence="ECO:0000305"
FT CONFLICT 193
FT /note="R -> L (in Ref. 1; BAA88556)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 226 AA; 25000 MW; 66DFBFB9B075F1AC CRC64;
MDLDADFLLL HLSDSALPTG AFAHSFGFET YMDAERITNA EEFQDWLKVL LKVQLTSSDA
LAMRMFYATP TVSELKRLDE RLFAGTPARE IREANARMGT RMAEIVAETY SVPLIVEYLE
LIQHRELSGH PALALALATH SAGIDVDRAI HAHLTATVSS LIQNAVRGIP LGQMAGQRVM
FAMREHIGAA VKRSANLDEI DFCSGDPGLD ISQMVHETQR ARLFMS
