ID   UREF_CUPNH              Reviewed;         230 AA.
AC   Q0KCP4; O30339;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=H16_A1086;
OS   Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442
OS   / H16 / Stanier 337) (Ralstonia eutropha).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=381666;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Piettre C., Toussaint A.;
RT   "Ralstonia eutropha H16 urease genes and proteins.";
RL   Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337;
RX   PubMed=16964242; DOI=10.1038/nbt1244;
RA   Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R.,
RA   Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I.,
RA   Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.;
RT   "Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia
RT   eutropha H16.";
RL   Nat. Biotechnol. 24:1257-1262(2006).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Y13732; CAA74069.1; -; Genomic_DNA.
DR   EMBL; AM260479; CAJ92227.1; -; Genomic_DNA.
DR   RefSeq; WP_011614869.1; NZ_CP039287.1.
DR   AlphaFoldDB; Q0KCP4; -.
DR   SMR; Q0KCP4; -.
DR   STRING; 381666.H16_A1086; -.
DR   EnsemblBacteria; CAJ92227; CAJ92227; H16_A1086.
DR   GeneID; 57643186; -.
DR   KEGG; reh:H16_A1086; -.
DR   PATRIC; fig|381666.6.peg.1470; -.
DR   eggNOG; COG0830; Bacteria.
DR   HOGENOM; CLU_049215_2_1_4; -.
DR   OMA; TQYTRLF; -.
DR   OrthoDB; 1942614at2; -.
DR   Proteomes; UP000008210; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion; Reference proteome.
FT   CHAIN           1..230
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_0000344159"
FT   CONFLICT        45
FT                   /note="E -> D (in Ref. 2; CAA74069)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   230 AA;  25444 MW;  CA7C574B13142E0C CRC64;
     MTQLHQLISL LHLASPSLPI GGFSYSQGLE AAIDCGSVHD AASAERWIRD NLLHVQAQCE
     APLWLLLHRC WKAGDAAQVR TWNDWFRATR ETSELRLETE QMGWSLSRLI AQMEWGAPVL
     RETLAALSPV CLPTAFTAAC VALQVEARDG LAAYCFNWAE NQVAAAIKAV PLGQVAGQHM
     LRRLHCAVLD TVDEATRRAD ATPPQLSTFS PMLGLLSARH ETQYSRLFRS