ID   UREF_ECO57              Reviewed;         224 AA.
AC   Q8X9U5; Q7AFH3;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF1 {ECO:0000255|HAMAP-Rule:MF_01385};
GN   OrderedLocusNames=Z1147, ECs1326;
GN   and
GN   Name=ureF2 {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=Z1586;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
RN   [3]
RP   ABSENCE OF UREASE.
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=15470125; DOI=10.1099/mic.0.27280-0;
RA   Nakano M., Iida T., Honda T.;
RT   "Urease activity of enterohaemorrhagic Escherichia coli depends on a
RT   specific one-base substitution in ureD.";
RL   Microbiology 150:3483-3489(2004).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- CAUTION: Neither O157 strain expresses urease due to a truncation of
CC       ureD, the last gene of the probable operon. Urease activity is restored
CC       in O157 / Sakai upon complementation with wild-type ureD.
CC       {ECO:0000305}.
CC   -!- CAUTION: This region of the chromosome is duplicated in strain O157:H7
CC       / EDL933 but not in O157:H7 / Sakai. {ECO:0000305}.
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DR   EMBL; AE005174; AAG55292.1; -; Genomic_DNA.
DR   EMBL; AE005174; AAG55701.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB34749.1; -; Genomic_DNA.
DR   PIR; A85655; A85655.
DR   PIR; F90794; F90794.
DR   RefSeq; NP_309353.1; NC_002695.1.
DR   RefSeq; WP_001142971.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; Q8X9U5; -.
DR   SMR; Q8X9U5; -.
DR   STRING; 155864.EDL933_1046; -.
DR   EnsemblBacteria; AAG55292; AAG55292; Z1147.
DR   EnsemblBacteria; AAG55701; AAG55701; Z1586.
DR   EnsemblBacteria; BAB34749; BAB34749; ECs_1326.
DR   GeneID; 912425; -.
DR   KEGG; ece:Z1147; -.
DR   KEGG; ece:Z1586; -.
DR   KEGG; ecs:ECs_1326; -.
DR   PATRIC; fig|386585.9.peg.1431; -.
DR   eggNOG; COG0830; Bacteria.
DR   HOGENOM; CLU_049215_2_1_6; -.
DR   OMA; TQYTRLF; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion; Reference proteome.
FT   CHAIN           1..224
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_0000344122"
SQ   SEQUENCE   224 AA;  25156 MW;  996BDCC16DA53E5A CRC64;
     MPTPEKRLRL MQLASNSLPV GGYSWSQGLE WAVEAGWVED SAAFEHWQQL QMEQSFFAVD
     LPLLARLYRA CEAGDPDSAG RWTAYLLACR ETRELRDEER NRGAAFTRLL VDWQPDCPAE
     WRKLCQQSQL TGMAWLGVRW QISVSDLALS LGYSWIESAV MAGVRLVPYG QLAAQQLIMR
     LCARYAANMD SALATPDHAI GSATPLASIA SARHETQYSR LFRS