ID   UREF_ECOLX              Reviewed;          60 AA.
AC   Q03286;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Urease accessory protein UreF;
DE   Flags: Fragment;
GN   Name=ureF;
OS   Escherichia coli.
OG   Plasmid.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC   STRAIN=1440 / UPEC;
RX   PubMed=8449894; DOI=10.1128/jb.175.6.1860-1864.1993;
RA   D'Orazio S.E., Collins C.M.;
RT   "Characterization of a plasmid-encoded urease gene cluster found in members
RT   of the family Enterobacteriaceae.";
RL   J. Bacteriol. 175:1860-1864(1993).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000250}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- INDUCTION: The probable operon is induced by urea.
CC       {ECO:0000269|PubMed:8449894}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000305}.
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DR   EMBL; L03308; AAA24748.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q03286; -.
DR   SMR; Q03286; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
PE   2: Evidence at transcript level;
KW   Chaperone; Cytoplasm; Nickel insertion; Plasmid.
FT   CHAIN           <1..60
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_0000067646"
FT   NON_TER         1
SQ   SEQUENCE   60 AA;  6654 MW;  84E21A81AEC0A4F5 CRC64;
     KLIPLGQSAG QNILFTLAER IPEVVAQSAI WPLDDIGSFT PAQIIASSRH ETQYTRLFRS