UREF_HELPY
ID UREF_HELPY Reviewed; 254 AA.
AC Q09065;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=HP_0069;
OS Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=85962;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RC STRAIN=85P;
RX PubMed=1313413; DOI=10.1128/jb.174.8.2466-2473.1992;
RA Cussac V., Ferrero R.L., Labigne A.;
RT "Expression of Helicobacter pylori urease genes in Escherichia coli grown
RT under nitrogen-limiting conditions.";
RL J. Bacteriol. 174:2466-2473(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700392 / 26695;
RX PubMed=9252185; DOI=10.1038/41483;
RA Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT "The complete genome sequence of the gastric pathogen Helicobacter
RT pylori.";
RL Nature 388:539-547(1997).
RN [3]
RP REVIEW ON VIRULENCE OF H.PYLORI.
RX PubMed=17313591; DOI=10.1111/j.1574-6968.2007.00648.x;
RA Clyne M., Dolan B., Reeves E.P.;
RT "Bacterial factors that mediate colonization of the stomach and virulence
RT of Helicobacter pylori.";
RL FEMS Microbiol. Lett. 268:135-143(2007).
CC -!- FUNCTION: Required for maturation of urease via the functional
CC incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC Rule:MF_01385}.
CC -!- SUBUNIT: UreH, UreF and UreG form a complex that acts as a GTP-
CC hydrolysis-dependent molecular chaperone, activating the urease
CC apoprotein by helping to assemble the nickel containing metallocenter
CC of UreC. The UreE protein probably delivers the nickel.
CC {ECO:0000255|HAMAP-Rule:MF_01385}.
CC -!- INTERACTION:
CC Q09065; Q09066: ureG; NbExp=6; IntAct=EBI-7743673, EBI-7742750;
CC Q09065; Q09067: ureH; NbExp=12; IntAct=EBI-7743673, EBI-7718225;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC -!- DISRUPTION PHENOTYPE: Cells do not express urease.
CC {ECO:0000269|PubMed:1313413}.
CC -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC Rule:MF_01385}.
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DR EMBL; M84338; AAA25024.1; -; Genomic_DNA.
DR EMBL; AE000511; AAD07132.1; -; Genomic_DNA.
DR PIR; E64528; E64528.
DR RefSeq; NP_206869.1; NC_000915.1.
DR RefSeq; WP_000357404.1; NC_018939.1.
DR PDB; 2WGL; X-ray; 2.00 A; A/B/C=1-254.
DR PDB; 3CXN; X-ray; 1.55 A; A/B/C=2-254.
DR PDB; 3O1Q; X-ray; 1.85 A; A/B/C=1-254.
DR PDB; 3SF5; X-ray; 2.50 A; A/C=1-254.
DR PDB; 4HI0; X-ray; 2.35 A; A/C=1-254.
DR PDBsum; 2WGL; -.
DR PDBsum; 3CXN; -.
DR PDBsum; 3O1Q; -.
DR PDBsum; 3SF5; -.
DR PDBsum; 4HI0; -.
DR AlphaFoldDB; Q09065; -.
DR SMR; Q09065; -.
DR DIP; DIP-3134N; -.
DR IntAct; Q09065; 5.
DR MINT; Q09065; -.
DR STRING; 85962.C694_00330; -.
DR PaxDb; Q09065; -.
DR DNASU; 900170; -.
DR EnsemblBacteria; AAD07132; AAD07132; HP_0069.
DR KEGG; hpy:HP_0069; -.
DR PATRIC; fig|85962.47.peg.72; -.
DR eggNOG; COG0830; Bacteria.
DR OMA; TQYTRLF; -.
DR PhylomeDB; Q09065; -.
DR BioCyc; MetaCyc:HP_RS00350-MON; -.
DR EvolutionaryTrace; Q09065; -.
DR Proteomes; UP000000429; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IMP:CACAO.
DR Gene3D; 1.10.4190.10; -; 1.
DR HAMAP; MF_01385; UreF; 1.
DR InterPro; IPR002639; UreF.
DR InterPro; IPR038277; UreF_sf.
DR Pfam; PF01730; UreF; 1.
DR PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chaperone; Cytoplasm; Nickel insertion; Reference proteome;
KW Virulence.
FT CHAIN 1..254
FT /note="Urease accessory protein UreF"
FT /id="PRO_0000067648"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 10
FT /note="T -> I (in Ref. 1; AAA25024)"
FT /evidence="ECO:0000305"
FT CONFLICT 17
FT /note="P -> L (in Ref. 1; AAA25024)"
FT /evidence="ECO:0000305"
FT CONFLICT 25
FT /note="N -> S (in Ref. 1; AAA25024)"
FT /evidence="ECO:0000305"
FT CONFLICT 55..60
FT /note="ETYIQQ -> LARNLHPA (in Ref. 1; AAA25024)"
FT /evidence="ECO:0000305"
FT CONFLICT 71
FT /note="E -> K (in Ref. 1; AAA25024)"
FT /evidence="ECO:0000305"
FT CONFLICT 103
FT /note="K -> R (in Ref. 1; AAA25024)"
FT /evidence="ECO:0000305"
FT CONFLICT 110..112
FT /note="VIM -> IIT (in Ref. 1; AAA25024)"
FT /evidence="ECO:0000305"
FT CONFLICT 143..145
FT /note="MGE -> IGA (in Ref. 1; AAA25024)"
FT /evidence="ECO:0000305"
FT CONFLICT 153..155
FT /note="KTK -> QTE (in Ref. 1; AAA25024)"
FT /evidence="ECO:0000305"
FT CONFLICT 232
FT /note="T -> A (in Ref. 1; AAA25024)"
FT /evidence="ECO:0000305"
FT TURN 25..28
FT /evidence="ECO:0007829|PDB:3O1Q"
FT HELIX 31..39
FT /evidence="ECO:0007829|PDB:3CXN"
FT STRAND 43..45
FT /evidence="ECO:0007829|PDB:3CXN"
FT HELIX 47..49
FT /evidence="ECO:0007829|PDB:4HI0"
FT HELIX 51..59
FT /evidence="ECO:0007829|PDB:3CXN"
FT HELIX 66..77
FT /evidence="ECO:0007829|PDB:3CXN"
FT HELIX 80..83
FT /evidence="ECO:0007829|PDB:3CXN"
FT HELIX 85..97
FT /evidence="ECO:0007829|PDB:3CXN"
FT HELIX 101..114
FT /evidence="ECO:0007829|PDB:3CXN"
FT HELIX 118..137
FT /evidence="ECO:0007829|PDB:3CXN"
FT HELIX 144..153
FT /evidence="ECO:0007829|PDB:3CXN"
FT HELIX 159..170
FT /evidence="ECO:0007829|PDB:3CXN"
FT HELIX 174..196
FT /evidence="ECO:0007829|PDB:3CXN"
FT HELIX 201..210
FT /evidence="ECO:0007829|PDB:3CXN"
FT HELIX 212..224
FT /evidence="ECO:0007829|PDB:3CXN"
FT HELIX 227..229
FT /evidence="ECO:0007829|PDB:3CXN"
FT HELIX 236..243
FT /evidence="ECO:0007829|PDB:4HI0"
FT HELIX 244..246
FT /evidence="ECO:0007829|PDB:4HI0"
SQ SEQUENCE 254 AA; 28619 MW; 6209F547A96FE028 CRC64;
MDKGKSVKST EKSVGMPPKT PKTDNNAHVD NEFLILQVND AVFPIGSYTH SFGLETYIQQ
KKVTNKESAL EYLKANLSSQ FLYTEMLSLK LTYESALQQD LKKILGVEEV IMLSTSPMEL
RLANQKLGNR FIKTLQAMNE LDMGEFFNAY AQKTKDPTHA TSYGVFAASL GIELKKALRH
YLYAQTSNMV INCVKSVPLS QNDGQKILLS LQSPFNQLIE KTLELDESHL CTASVQNDIK
AMQHESLYSR LYMS
