ID   UREF_KLEAE              Reviewed;         224 AA.
AC   P18318;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385};
OS   Klebsiella aerogenes (Enterobacter aerogenes).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=548;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CG253;
RX   PubMed=2211515; DOI=10.1128/jb.172.10.5837-5843.1990;
RA   Mulrooney S.B., Hausinger R.P.;
RT   "Sequence of the Klebsiella aerogenes urease genes and evidence for
RT   accessory proteins facilitating nickel incorporation.";
RL   J. Bacteriol. 172:5837-5843(1990).
RN   [2]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=1624427; DOI=10.1128/jb.174.13.4324-4330.1992;
RA   Lee M.H., Mulrooney S.B., Renner M.J., Markowicz Y., Hausinger R.P.;
RT   "Klebsiella aerogenes urease gene cluster: sequence of ureD and
RT   demonstration that four accessory genes (ureD, ureE, ureF, and ureG) are
RT   involved in nickel metallocenter biosynthesis.";
RL   J. Bacteriol. 174:4324-4330(1992).
RN   [3]
RP   COMPLEX FORMATION, AND INTERACTION WITH UREA; UREB; UREC; URED AND UREG.
RX   PubMed=10500143; DOI=10.1073/pnas.96.20.11140;
RA   Soriano A., Hausinger R.P.;
RT   "GTP-dependent activation of urease apoprotein in complex with the UreD,
RT   UreF, and UreG accessory proteins.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:11140-11144(1999).
RN   [4]
RP   INTERACTION WITH UREB, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=14749331; DOI=10.1074/jbc.m312979200;
RA   Chang Z., Kuchar J., Hausinger R.P.;
RT   "Chemical cross-linking and mass spectrometric identification of sites of
RT   interaction for UreD, UreF, and urease.";
RL   J. Biol. Chem. 279:15305-15313(2004).
RN   [5]
RP   UREE-UREF FUSION PROTEIN, AND DELETION OF THE C-TERMINUS.
RX   PubMed=17041056; DOI=10.1128/jb.01265-06;
RA   Kim J.K., Mulrooney S.B., Hausinger R.P.;
RT   "The UreEF fusion protein provides a soluble and functional form of the
RT   UreF urease accessory protein.";
RL   J. Bacteriol. 188:8413-8420(2006).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC   -!- INTERACTION:
CC       P18318; Q09063: ureD; NbExp=4; IntAct=EBI-6410604, EBI-6410589;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Cells do not express urease.
CC       {ECO:0000269|PubMed:1624427}.
CC   -!- MISCELLANEOUS: The artificial UreEF fusion protein binds to UreD-UreABC
CC       apoprotein complex, and UreG can also bind to this. The UreF portion
CC       seems to function normally in nickel transfer, while the UreE function
CC       is impaired in the fusion.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; M36068; AAA25153.1; -; Genomic_DNA.
DR   AlphaFoldDB; P18318; -.
DR   SMR; P18318; -.
DR   IntAct; P18318; 1.
DR   PRIDE; P18318; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   1: Evidence at protein level;
KW   Chaperone; Cytoplasm; Nickel insertion.
FT   CHAIN           1..224
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_0000067650"
FT   REGION          210..224
FT                   /note="Required for interaction with the urease apoprotein-
FT                   UreDG complex"
SQ   SEQUENCE   224 AA;  25222 MW;  4A02E5F4D7AD7C32 CRC64;
     MSTAEQRLRL MQLASSNLPV GGYSWSQGLE WAVEAGWVLD VAAFERWQRR QMTEGFFTVD
     LPLFARLYRA CEQGDIAAAQ RWTAYLLACR ETRELREEER NRGAAFARLL SDWQPDCPPP
     WRSLCQQSQL AGMAWLGVRW RIALPEMALS LGYSWIESAV MAGVKLVPFG QQAAQQLILR
     LCDHYAAEMP RALAAPDGDI GSATPLAAIA SARHETQYSR LFRS