ID   UREF_KLEP7              Reviewed;         224 AA.
AC   A6TE44;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   OrderedLocusNames=KPN78578_34040; ORFNames=KPN_03469;
OS   Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=272620;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700721 / MGH 78578;
RG   The Klebsiella pneumonia Genome Sequencing Project;
RA   McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P.,
RA   Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K.;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; CP000647; ABR78865.1; -; Genomic_DNA.
DR   RefSeq; WP_015959000.1; NC_009648.1.
DR   PDB; 6JC4; X-ray; 2.30 A; A/B/C/D=1-224.
DR   PDBsum; 6JC4; -.
DR   AlphaFoldDB; A6TE44; -.
DR   SMR; A6TE44; -.
DR   STRING; 272620.KPN_03469; -.
DR   EnsemblBacteria; ABR78865; ABR78865; KPN_03469.
DR   KEGG; kpn:KPN_03469; -.
DR   HOGENOM; CLU_049215_2_1_6; -.
DR   OMA; TQYTRLF; -.
DR   Proteomes; UP000000265; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Chaperone; Cytoplasm; Nickel insertion; Reference proteome.
FT   CHAIN           1..224
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_0000344128"
FT   HELIX           4..14
FT                   /evidence="ECO:0007829|PDB:6JC4"
FT   TURN            24..26
FT                   /evidence="ECO:0007829|PDB:6JC4"
FT   HELIX           27..35
FT                   /evidence="ECO:0007829|PDB:6JC4"
FT   HELIX           41..54
FT                   /evidence="ECO:0007829|PDB:6JC4"
FT   HELIX           55..59
FT                   /evidence="ECO:0007829|PDB:6JC4"
FT   HELIX           60..72
FT                   /evidence="ECO:0007829|PDB:6JC4"
FT   HELIX           76..89
FT                   /evidence="ECO:0007829|PDB:6JC4"
FT   HELIX           93..113
FT                   /evidence="ECO:0007829|PDB:6JC4"
FT   HELIX           119..125
FT                   /evidence="ECO:0007829|PDB:6JC4"
FT   HELIX           129..140
FT                   /evidence="ECO:0007829|PDB:6JC4"
FT   HELIX           144..166
FT                   /evidence="ECO:0007829|PDB:6JC4"
FT   HELIX           171..194
FT                   /evidence="ECO:0007829|PDB:6JC4"
FT   HELIX           197..199
FT                   /evidence="ECO:0007829|PDB:6JC4"
SQ   SEQUENCE   224 AA;  25236 MW;  5A4EB9F887F57177 CRC64;
     MSTAEQRLRL MQLASSNLPV GGYSWSQGLE WAVEAGWVPD VAAFERWQRR QMTEGFFTVD
     LPLFARLYRA CEQGDIAAAQ RWTAYLLACR ETRELREEER NRGAAFARLL SDWQPDCPPP
     WRSLCQQSQL AGMAWLGVRW RIALPEMALS LGYSWIESAV MAGVKLVPFG QQAAQQLILR
     LCDHYAAEMP RALATPDGDI GSATPLAAIA SARHETQYSR LFRS