ID   UREF_MYCTU              Reviewed;         211 AA.
AC   P9WFE5; L0T7W1; P50050;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=Rv1851;
GN   ORFNames=MTCY359.22c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX   PubMed=7559354; DOI=10.1128/jb.177.19.5644-5652.1995;
RA   Clemens D.L., Lee B.-Y., Horwitz M.A.;
RT   "Purification, characterization, and genetic analysis of Mycobacterium
RT   tuberculosis urease, a potentially critical determinant of host-pathogen
RT   interaction.";
RL   J. Bacteriol. 177:5644-5652(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; U33011; AAC43476.1; -; Genomic_DNA.
DR   EMBL; AL123456; CCP44617.1; -; Genomic_DNA.
DR   PIR; C70665; C70665.
DR   RefSeq; NP_216367.1; NC_000962.3.
DR   RefSeq; WP_003899050.1; NZ_NVQJ01000013.1.
DR   AlphaFoldDB; P9WFE5; -.
DR   SMR; P9WFE5; -.
DR   STRING; 83332.Rv1851; -.
DR   PaxDb; P9WFE5; -.
DR   DNASU; 885532; -.
DR   GeneID; 885532; -.
DR   KEGG; mtu:Rv1851; -.
DR   TubercuList; Rv1851; -.
DR   eggNOG; COG0830; Bacteria.
DR   OMA; HCVAYET; -.
DR   PhylomeDB; P9WFE5; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion; Reference proteome.
FT   CHAIN           1..211
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_0000067651"
FT   REGION          71..93
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   211 AA;  22356 MW;  8B2934BCC1E1CCB7 CRC64;
     MTSLAVLLTL ADSRLPTGAH VHSGGIEEAI AAGMVTGLAT LEAFLKRRVR THGLLTASIA
     AAVHRGELAV DDADRETDAR TPAPAARHAS RSQGRGLIRL ARRVWPDSGW EELGPRPHLA
     VVAGRVGALS GLAPEHNALH LVYITMTGSA IAAQRLLALD PAEVTVVTFQ LSELCEQIAQ
     EATAGLADLS DPLLDTLAQR HDERVRPLFV S