ID   UREF_PSEAB              Reviewed;         223 AA.
AC   Q02FC6;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=PA14_64660;
OS   Pseudomonas aeruginosa (strain UCBPP-PA14).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCBPP-PA14;
RX   PubMed=17038190; DOI=10.1186/gb-2006-7-10-r90;
RA   Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S.,
RA   Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G.,
RA   Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.;
RT   "Genomic analysis reveals that Pseudomonas aeruginosa virulence is
RT   combinatorial.";
RL   Genome Biol. 7:R90.1-R90.14(2006).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; CP000438; ABJ14277.1; -; Genomic_DNA.
DR   RefSeq; WP_003095528.1; NZ_CP034244.1.
DR   AlphaFoldDB; Q02FC6; -.
DR   SMR; Q02FC6; -.
DR   PRIDE; Q02FC6; -.
DR   EnsemblBacteria; ABJ14277; ABJ14277; PA14_64660.
DR   KEGG; pau:PA14_64660; -.
DR   HOGENOM; CLU_049215_2_1_6; -.
DR   OMA; TQYTRLF; -.
DR   BioCyc; PAER208963:G1G74-5464-MON; -.
DR   Proteomes; UP000000653; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion.
FT   CHAIN           1..223
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_0000344148"
SQ   SEQUENCE   223 AA;  24636 MW;  1BC7BE5643529945 CRC64;
     MNSIWARLRL ASSQLPIGGY SYSQGLEAAL DNGWVRDAES ARTWLVDQLQ LNLARFEAPL
     LAGLLRAALI GDWAACDAAS ERHRASRETR ELAQESRQMG FSLHQLLEAL PELDAPGRAW
     LARQDPPNLA AAWAMAARAW RLDAEEALSA WFWSWLENQL AVLMKTLPLG QLAAQKLASS
     LLPELDRACA EALRRPAVEG SAAFGLALAS MTHETQYSRL FRS