ID   UREF_PSEAE              Reviewed;         223 AA.
AC   Q9HUS1;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=PA4892;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; AE004091; AAG08277.1; -; Genomic_DNA.
DR   PIR; F83034; F83034.
DR   RefSeq; NP_253579.1; NC_002516.2.
DR   RefSeq; WP_003095528.1; NZ_QZGE01000002.1.
DR   AlphaFoldDB; Q9HUS1; -.
DR   SMR; Q9HUS1; -.
DR   STRING; 287.DR97_2243; -.
DR   PaxDb; Q9HUS1; -.
DR   DNASU; 882295; -.
DR   EnsemblBacteria; AAG08277; AAG08277; PA4892.
DR   GeneID; 882295; -.
DR   KEGG; pae:PA4892; -.
DR   PATRIC; fig|208964.12.peg.5125; -.
DR   PseudoCAP; PA4892; -.
DR   HOGENOM; CLU_049215_2_1_6; -.
DR   InParanoid; Q9HUS1; -.
DR   OMA; TQYTRLF; -.
DR   PhylomeDB; Q9HUS1; -.
DR   BioCyc; PAER208964:G1FZ6-5006-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion; Reference proteome.
FT   CHAIN           1..223
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_0000287730"
SQ   SEQUENCE   223 AA;  24636 MW;  1BC7BE5643529945 CRC64;
     MNSIWARLRL ASSQLPIGGY SYSQGLEAAL DNGWVRDAES ARTWLVDQLQ LNLARFEAPL
     LAGLLRAALI GDWAACDAAS ERHRASRETR ELAQESRQMG FSLHQLLEAL PELDAPGRAW
     LARQDPPNLA AAWAMAARAW RLDAEEALSA WFWSWLENQL AVLMKTLPLG QLAAQKLASS
     LLPELDRACA EALRRPAVEG SAAFGLALAS MTHETQYSRL FRS