ID   UREF_PSEP1              Reviewed;         224 AA.
AC   A5W4B3;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=Pput_2841;
OS   Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=351746;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700007 / DSM 6899 / BCRC 17059 / F1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P.;
RT   "Complete sequence of Pseudomonas putida F1.";
RL   Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; CP000712; ABQ78973.1; -; Genomic_DNA.
DR   RefSeq; WP_012052572.1; NC_009512.1.
DR   AlphaFoldDB; A5W4B3; -.
DR   SMR; A5W4B3; -.
DR   STRING; 351746.Pput_2841; -.
DR   EnsemblBacteria; ABQ78973; ABQ78973; Pput_2841.
DR   KEGG; ppf:Pput_2841; -.
DR   eggNOG; COG0830; Bacteria.
DR   HOGENOM; CLU_049215_2_1_6; -.
DR   OMA; TQYTRLF; -.
DR   OrthoDB; 1942614at2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion.
FT   CHAIN           1..224
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_0000344151"
SQ   SEQUENCE   224 AA;  24460 MW;  5AF4AF87EFEBAD24 CRC64;
     MNSDLALLRL LQLASPGLPV GGFTYSQGLE WAVEAGWVRG VDSFVGWQRE QVHDTLACLD
     WPVLARLYRA CQAEDAEAFG HWSRFLLANR ETAELRLEEQ QRGAALARLL DGWQLGQAPA
     WRASLELTQL GGMAWLAAHW AIPLRQLALG HGFAWLEGAV MAGVKLVPFG QQAAQTLLRD
     LGADLPAALD QALALGDDQL GGGLPLLAIA SSRHETQYTR LFRS