UREF_PSEPW
ID UREF_PSEPW Reviewed; 224 AA.
AC B1J818;
DT 22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 1.
DT 25-MAY-2022, entry version 62.
DE RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385};
GN OrderedLocusNames=PputW619_2420;
OS Pseudomonas putida (strain W619).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=390235;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=W619;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S.,
RA Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L.,
RA Kyrpides N., Kim E., Taghavi S., Vangronsveld D., van der Lelie D.,
RA Richardson P.;
RT "Complete sequence of Pseudomonas putida W619.";
RL Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Required for maturation of urease via the functional
CC incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC Rule:MF_01385}.
CC -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC hydrolysis-dependent molecular chaperone, activating the urease
CC apoprotein by helping to assemble the nickel containing metallocenter
CC of UreC. The UreE protein probably delivers the nickel.
CC {ECO:0000255|HAMAP-Rule:MF_01385}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC Rule:MF_01385}.
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DR EMBL; CP000949; ACA72920.1; -; Genomic_DNA.
DR RefSeq; WP_012314280.1; NC_010501.1.
DR AlphaFoldDB; B1J818; -.
DR SMR; B1J818; -.
DR STRING; 390235.PputW619_2420; -.
DR EnsemblBacteria; ACA72920; ACA72920; PputW619_2420.
DR KEGG; ppw:PputW619_2420; -.
DR eggNOG; COG0830; Bacteria.
DR HOGENOM; CLU_049215_2_1_6; -.
DR OMA; TQYTRLF; -.
DR OrthoDB; 1942614at2; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR Gene3D; 1.10.4190.10; -; 1.
DR HAMAP; MF_01385; UreF; 1.
DR InterPro; IPR002639; UreF.
DR InterPro; IPR038277; UreF_sf.
DR Pfam; PF01730; UreF; 1.
DR PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE 3: Inferred from homology;
KW Chaperone; Cytoplasm; Nickel insertion.
FT CHAIN 1..224
FT /note="Urease accessory protein UreF"
FT /id="PRO_0000344154"
SQ SEQUENCE 224 AA; 24768 MW; 7232EE380587C724 CRC64;
MNSDLALLRL LQLASPGLPV GGFTYSQGLE WAVEAGWVRD IKSFSAWQRE QMNDTLAYLD
WPVLARLYYA CKADDADAFA RWSRFLLANR ETAELRLEEQ QRGAALARLL DGWQLGQAPA
WRPSLELSQL GGMAWLAVHW SIPLRQLALG HAFAWLEGAV MAGVKLVPFG QQAAQTLLRD
LGEILPGVID QALALGDDQL GGGLPLLAIA SSRHETQYTR LFRS