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CADB_ECOLI
ID   CADB_ECOLI              Reviewed;         444 AA.
AC   P0AAE8; P23891; Q2M6H1;
DT   11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=Cadaverine/lysine antiporter {ECO:0000305};
GN   Name=cadB {ECO:0000303|PubMed:1556085}; OrderedLocusNames=b4132, JW4093;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC   STRAIN=JLS821;
RX   PubMed=1370290; DOI=10.1128/jb.174.2.530-540.1992;
RA   Watson N., Dunyak D.S., Rosey E.L., Slonczewski J.L., Olson E.R.;
RT   "Identification of elements involved in transcriptional regulation of the
RT   Escherichia coli cad operon by external pH.";
RL   J. Bacteriol. 174:530-540(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=1556085; DOI=10.1128/jb.174.8.2659-2669.1992;
RA   Meng S.-Y., Bennett G.N.;
RT   "Nucleotide sequence of the Escherichia coli cad operon: a system for
RT   neutralization of low extracellular pH.";
RL   J. Bacteriol. 174:2659-2669(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA   Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT   "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT   from 92.8 through 100 minutes.";
RL   Nucleic Acids Res. 23:2105-2119(1995).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [6]
RP   FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL
RP   PROPERTIES, AND INDUCTION.
RX   PubMed=14982633; DOI=10.1046/j.1365-2958.2003.03913.x;
RA   Soksawatmaekhin W., Kuraishi A., Sakata K., Kashiwagi K., Igarashi K.;
RT   "Excretion and uptake of cadaverine by CadB and its physiological functions
RT   in Escherichia coli.";
RL   Mol. Microbiol. 51:1401-1412(2004).
RN   [7]
RP   INDUCTION.
RX   PubMed=16491024; DOI=10.1159/000090346;
RA   Kuper C., Jung K.;
RT   "CadC-mediated activation of the cadBA promoter in Escherichia coli.";
RL   J. Mol. Microbiol. Biotechnol. 10:26-39(2005).
RN   [8]
RP   SUBCELLULAR LOCATION, AND TOPOLOGY.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
RN   [9]
RP   BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TOPOLOGY, DOMAIN, AND
RP   MUTAGENESIS OF CYS-12; TRP-41; TRP-43; TYR-55; TYR-57; TYR-73; GLU-76;
RP   TYR-89; TYR-90; TYR-107; CYS-125; TYR-174; ASP-185; CYS-196; GLU-204;
RP   TYR-235; TYR-246; CYS-282; ARG-299; ASP-303; TYR-310; TYR-366; TYR-368;
RP   CYS-370; GLU-377; CYS-389; CYS-394; CYS-397; GLU-408 AND TYR-423.
RX   PubMed=16877381; DOI=10.1074/jbc.m600754200;
RA   Soksawatmaekhin W., Uemura T., Fukiwake N., Kashiwagi K., Igarashi K.;
RT   "Identification of the cadaverine recognition site on the cadaverine-lysine
RT   antiporter CadB.";
RL   J. Biol. Chem. 281:29213-29220(2006).
RN   [10]
RP   TRANSCRIPTIONAL REGULATION BY LRP.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=21441513; DOI=10.1128/jb.00815-10;
RA   Ruiz J., Haneburger I., Jung K.;
RT   "Identification of ArgP and Lrp as transcriptional regulators of lysP, the
RT   gene encoding the specific lysine permease of Escherichia coli.";
RL   J. Bacteriol. 193:2536-2548(2011).
RN   [11]
RP   REVIEW.
RX   PubMed=21796432; DOI=10.1007/s00726-011-0989-9;
RA   Tomitori H., Kashiwagi K., Igarashi K.;
RT   "Structure and function of polyamine-amino acid antiporters CadB and PotE
RT   in Escherichia coli.";
RL   Amino Acids 42:733-740(2012).
RN   [12]
RP   INDUCTION.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=29138484; DOI=10.1038/s41467-017-02030-0;
RA   Chakraborty S., Winardhi R.S., Morgan L.K., Yan J., Kenney L.J.;
RT   "Non-canonical activation of OmpR drives acid and osmotic stress responses
RT   in single bacterial cells.";
RL   Nat. Commun. 8:1587-1587(2017).
CC   -!- FUNCTION: Under acidic conditions, in the presence of lysine, functions
CC       as a cadaverine:lysine antiporter that facilitates the excretion of
CC       cadaverine and the uptake of lysine (PubMed:1556085, PubMed:14982633).
CC       At neutral pH, also catalyzes the uptake of cadaverine via a proton
CC       symport mechanism, however the physiological relevance of this uptake
CC       activity is probably negligible because the expression of cadB is low
CC       at neutral pH (PubMed:14982633). Cadaverine uptake activity is low at
CC       acidic pH (PubMed:14982633). {ECO:0000269|PubMed:14982633,
CC       ECO:0000269|PubMed:1556085}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cadaverine(in) + L-lysine(out) = cadaverine(out) + L-
CC         lysine(in); Xref=Rhea:RHEA:28895, ChEBI:CHEBI:32551,
CC         ChEBI:CHEBI:58384; Evidence={ECO:0000269|PubMed:14982633,
CC         ECO:0000305|PubMed:1556085};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:28896;
CC         Evidence={ECO:0000269|PubMed:14982633, ECO:0000305|PubMed:1556085};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cadaverine(in) + H(+)(in) = cadaverine(out) + H(+)(out);
CC         Xref=Rhea:RHEA:29711, ChEBI:CHEBI:15378, ChEBI:CHEBI:58384;
CC         Evidence={ECO:0000269|PubMed:14982633};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:29713;
CC         Evidence={ECO:0000269|PubMed:14982633};
CC   -!- ACTIVITY REGULATION: Uptake of cadaverine at neutral pH is greatly
CC       inhibited by carbonyl cyanide m-chlorophenylhydrazone (CCCP), which
CC       dissipates the proton motive force, valinomycin, which dissipates the
CC       membrane potential, and nigericin, which dissipates the transmembrane
CC       pH gradient (PubMed:14982633). Cadaverine uptake at neutral pH is also
CC       inhibited by putrescine and lysine (PubMed:14982633). Cadaverine-lysine
CC       antiporter activity is not inhibited by CCCP (PubMed:14982633).
CC       {ECO:0000269|PubMed:14982633}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=303 uM for cadaverine (for export activity, at low pH)
CC         {ECO:0000269|PubMed:14982633};
CC         KM=390 uM for cadaverine (for export activity)
CC         {ECO:0000269|PubMed:16877381};
CC         KM=20.8 uM for cadaverine (for uptake activity, at neutral pH)
CC         {ECO:0000269|PubMed:14982633, ECO:0000269|PubMed:16877381};
CC         Vmax=0.313 nmol/min/mg enzyme (for cadaverine export activity, at low
CC         pH) {ECO:0000269|PubMed:14982633};
CC         Vmax=11.8 nmol/min/mg enzyme (for cadaverine uptake activity, at
CC         neutral pH) {ECO:0000269|PubMed:14982633};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:15919996,
CC       ECO:0000269|PubMed:16877381}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- INDUCTION: Part of the cadB-cadA operon, which is under the control of
CC       the Pcad promoter (PubMed:1370290, PubMed:16491024). Expression is
CC       regulated by CadC (PubMed:1370290, PubMed:16491024). Highly expressed
CC       at acidic pH in the presence of lysine (PubMed:1370290,
CC       PubMed:16491024, PubMed:14982633). The global regulator Lrp has also a
CC       positive effect on the expression of the cadBA operon when cells are
CC       exposed to moderate acidic stress in the presence of lysine
CC       (PubMed:21441513). Repressed by H-NS under non-inducing conditions
CC       (PubMed:16491024). Repressed at pH 5.6 by OmpR (PubMed:29138484).
CC       {ECO:0000269|PubMed:1370290, ECO:0000269|PubMed:14982633,
CC       ECO:0000269|PubMed:16491024, ECO:0000269|PubMed:21441513,
CC       ECO:0000269|PubMed:29138484}.
CC   -!- DOMAIN: Amino acid residues involved in both uptake and excretion, or
CC       solely in excretion, are located in the cytoplasmic loops and the
CC       cytoplasmic side of transmembrane segments, whereas residues involved
CC       in uptake are located in the periplasmic loops and the transmembrane
CC       segments. The SH-group of Cys-370 seems to be important for both uptake
CC       and excretion and may be necessary for recognition of the NH(2)-group
CC       of cadaverine. {ECO:0000269|PubMed:16877381}.
CC   -!- DISRUPTION PHENOTYPE: Mutant shows reduced amount of cadaverine in the
CC       medium. {ECO:0000269|PubMed:1556085}.
CC   -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC       superfamily. Basic amino acid/polyamine antiporter (APA) (TC 2.A.3.2)
CC       family. {ECO:0000305}.
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DR   EMBL; M67452; AAA23532.1; -; Genomic_DNA.
DR   EMBL; M76411; AAA23535.1; -; Genomic_DNA.
DR   EMBL; U14003; AAA97032.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC77093.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE78135.1; -; Genomic_DNA.
DR   PIR; A41842; A41842.
DR   RefSeq; NP_418556.1; NC_000913.3.
DR   RefSeq; WP_000092909.1; NZ_STEB01000014.1.
DR   AlphaFoldDB; P0AAE8; -.
DR   SMR; P0AAE8; -.
DR   BioGRID; 4260780; 7.
DR   DIP; DIP-48089N; -.
DR   IntAct; P0AAE8; 2.
DR   STRING; 511145.b4132; -.
DR   TCDB; 2.A.3.2.2; the amino acid-polyamine-organocation (apc) family.
DR   PaxDb; P0AAE8; -.
DR   PRIDE; P0AAE8; -.
DR   EnsemblBacteria; AAC77093; AAC77093; b4132.
DR   EnsemblBacteria; BAE78135; BAE78135; BAE78135.
DR   GeneID; 66671956; -.
DR   GeneID; 948654; -.
DR   KEGG; ecj:JW4093; -.
DR   KEGG; eco:b4132; -.
DR   PATRIC; fig|1411691.4.peg.2567; -.
DR   EchoBASE; EB0130; -.
DR   eggNOG; COG0531; Bacteria.
DR   HOGENOM; CLU_007946_1_0_6; -.
DR   InParanoid; P0AAE8; -.
DR   OMA; FAYDGWL; -.
DR   PhylomeDB; P0AAE8; -.
DR   BioCyc; EcoCyc:CADB-MON; -.
DR   BioCyc; MetaCyc:CADB-MON; -.
DR   PRO; PR:P0AAE8; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR   GO; GO:0016020; C:membrane; IDA:EcoCyc.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR   GO; GO:0043872; F:lysine:cadaverine antiporter activity; IDA:EcoCyc.
DR   GO; GO:0015295; F:solute:proton symporter activity; IDA:EcoCyc.
DR   GO; GO:0015839; P:cadaverine transport; IDA:EcoCyc.
DR   GO; GO:1990451; P:cellular stress response to acidic pH; IEP:EcoCyc.
DR   GO; GO:1903401; P:L-lysine transmembrane transport; IDA:EcoCyc.
DR   InterPro; IPR002293; AA/rel_permease1.
DR   InterPro; IPR004754; Amino_acid_antiprt.
DR   Pfam; PF13520; AA_permease_2; 1.
DR   TIGRFAMs; TIGR00905; 2A0302; 1.
PE   1: Evidence at protein level;
KW   Amino-acid transport; Antiport; Cell inner membrane; Cell membrane;
KW   Membrane; Reference proteome; Symport; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..444
FT                   /note="Cadaverine/lysine antiporter"
FT                   /id="PRO_0000054244"
FT   TOPO_DOM        1..6
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:16877381"
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        28..34
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305|PubMed:16877381"
FT   TRANSMEM        35..55
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        56..94
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:16877381"
FT   TRANSMEM        95..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        116..122
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305|PubMed:16877381"
FT   TRANSMEM        123..143
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        144..148
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:16877381"
FT   TRANSMEM        149..169
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        170..192
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305|PubMed:16877381"
FT   TRANSMEM        193..213
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        214..221
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:16877381"
FT   TRANSMEM        222..242
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        243..272
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305|PubMed:16877381"
FT   TRANSMEM        273..293
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        294..322
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:16877381"
FT   TRANSMEM        323..343
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        344..353
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305|PubMed:16877381"
FT   TRANSMEM        354..374
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        375..383
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:16877381"
FT   TRANSMEM        384..404
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        405..425
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        426..444
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000269|PubMed:15919996,
FT                   ECO:0000305|PubMed:16877381"
FT   MUTAGEN         12
FT                   /note="C->S: Does not affect cadaverine excretion and
FT                   cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         41
FT                   /note="W->L: Moderate decrease in cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         43
FT                   /note="W->L: Strong decrease in cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         55
FT                   /note="Y->L: Moderate decrease in both cadaverine excretion
FT                   and cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         57
FT                   /note="Y->L: Strong decrease in cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         73
FT                   /note="Y->L: Strong decrease in both cadaverine excretion
FT                   and cadaverine uptake. 9-fold increase in Km for cadaverine
FT                   for cadaverine uptake and 10-fold increase in Km for
FT                   cadaverine for cadaverine excretion."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         76
FT                   /note="E->Q: Moderate decrease in both cadaverine excretion
FT                   and cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         89
FT                   /note="Y->L: Strong decrease in both cadaverine excretion
FT                   and cadaverine uptake. 10-fold increase in Km for
FT                   cadaverine for cadaverine uptake and 5-fold increase in Km
FT                   for cadaverine for cadaverine excretion."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         90
FT                   /note="Y->L: Strong decrease in both cadaverine excretion
FT                   and cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         107
FT                   /note="Y->L: Strong decrease in cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         125
FT                   /note="C->S: Does not affect cadaverine excretion and
FT                   cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         174
FT                   /note="Y->L: Moderate decrease in cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         185
FT                   /note="D->N: Moderate decrease in cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         196
FT                   /note="C->S: Moderate decrease in both cadaverine excretion
FT                   and cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         204
FT                   /note="E->Q: Strong decrease in both cadaverine excretion
FT                   and cadaverine uptake. 22-fold increase in Km for
FT                   cadaverine for cadaverine uptake and 6-fold increase in Km
FT                   for cadaverine for cadaverine excretion."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         235
FT                   /note="Y->L: Strong decrease in both cadaverine excretion
FT                   and cadaverine uptake. 23-fold increase in Km for
FT                   cadaverine for cadaverine uptake and 7-fold increase in Km
FT                   for cadaverine for cadaverine excretion."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         246
FT                   /note="Y->L: Moderate decrease in both cadaverine excretion
FT                   and cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         282
FT                   /note="C->S: Moderate decrease in both cadaverine excretion
FT                   and cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         299
FT                   /note="R->A: Strong decrease in cadaverine excretion but
FT                   not in cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         303
FT                   /note="D->N: Strong decrease in both cadaverine excretion
FT                   and cadaverine uptake. 24-fold increase in Km for
FT                   cadaverine for cadaverine uptake and 9-fold increase in Km
FT                   for cadaverine for cadaverine excretion."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         310
FT                   /note="Y->L: Moderate decrease in both cadaverine excretion
FT                   and cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         366
FT                   /note="Y->L: Strong decrease in cadaverine uptake. 15-fold
FT                   increase in Km for cadaverine for cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         368
FT                   /note="Y->L: Strong decrease in cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         370
FT                   /note="C->S: Strong decrease in both cadaverine excretion
FT                   and cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         377
FT                   /note="E->Q: Moderate decrease in both cadaverine excretion
FT                   and cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         389
FT                   /note="C->S: Moderate decrease in both cadaverine excretion
FT                   and cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         394
FT                   /note="C->S: Moderate decrease in both cadaverine excretion
FT                   and cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         397
FT                   /note="C->S: Moderate decrease in both cadaverine excretion
FT                   and cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         408
FT                   /note="E->Q: Moderate decrease in cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
FT   MUTAGEN         423
FT                   /note="Y->L: Strong decrease in both cadaverine excretion
FT                   and cadaverine uptake."
FT                   /evidence="ECO:0000269|PubMed:16877381"
SQ   SEQUENCE   444 AA;  46665 MW;  E87913B449B0500A CRC64;
     MSSAKKIGLF ACTGVVAGNM MGSGIALLPA NLASIGGIAI WGWIISIIGA MSLAYVYARL
     ATKNPQQGGP IAYAGEISPA FGFQTGVLYY HANWIGNLAI GITAVSYLST FFPVLNDPVP
     AGIACIAIVW VFTFVNMLGG TWVSRLTTIG LVLVLIPVVM TAIVGWHWFD AATYAANWNT
     ADTTDGHAII KSILLCLWAF VGVESAAVST GMVKNPKRTV PLATMLGTGL AGIVYIAATQ
     VLSGMYPSSV MAASGAPFAI SASTILGNWA APLVSAFTAF ACLTSLGSWM MLVGQAGVRA
     ANDGNFPKVY GEVDSNGIPK KGLLLAAVKM TALMILITLM NSAGGKASDL FGELTGIAVL
     LTMLPYFYSC VDLIRFEGVN IRNFVSLICS VLGCVFCFIA LMGASSFELA GTFIVSLIIL
     MFYARKMHER QSHSMDNHTA SNAH
 
 
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