ID   UREF_RHIL3              Reviewed;         223 AA.
AC   Q1MCW3;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=RL3727.1;
GN   ORFNames=RL3727A;
OS   Rhizobium leguminosarum bv. viciae (strain 3841).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=216596;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=3841;
RX   PubMed=16640791; DOI=10.1186/gb-2006-7-4-r34;
RA   Young J.P.W., Crossman L.C., Johnston A.W.B., Thomson N.R., Ghazoui Z.F.,
RA   Hull K.H., Wexler M., Curson A.R.J., Todd J.D., Poole P.S., Mauchline T.H.,
RA   East A.K., Quail M.A., Churcher C., Arrowsmith C., Cherevach I.,
RA   Chillingworth T., Clarke K., Cronin A., Davis P., Fraser A., Hance Z.,
RA   Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H.,
RA   Rabbinowitsch E., Sanders M., Simmonds M., Whitehead S., Parkhill J.;
RT   "The genome of Rhizobium leguminosarum has recognizable core and accessory
RT   components.";
RL   Genome Biol. 7:R34.1-R34.20(2006).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; AM236080; CAK09217.1; -; Genomic_DNA.
DR   RefSeq; WP_011653181.1; NC_008380.1.
DR   AlphaFoldDB; Q1MCW3; -.
DR   SMR; Q1MCW3; -.
DR   STRING; 216596.RL3727A; -.
DR   EnsemblBacteria; CAK09217; CAK09217; RL3727A.
DR   KEGG; rle:RL3727A; -.
DR   eggNOG; COG0830; Bacteria.
DR   HOGENOM; CLU_049215_2_0_5; -.
DR   OMA; TQYTRLF; -.
DR   OrthoDB; 1942614at2; -.
DR   Proteomes; UP000006575; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion.
FT   CHAIN           1..223
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_0000344165"
SQ   SEQUENCE   223 AA;  23555 MW;  721B342BB6284B0E CRC64;
     MTRDRELQAL LRLTAWLSPA FPIGSFAYSG GLERAVADGL VTDAALLAAW IGTLVGNGSV
     WNDAVLLAES HRQQAEAARL AEVAALAEAL AGSRERHQET MLLGEAFLAA ARAWPDEVFR
     RLPDKVAYPI AVGAVTGAHG IRPEKALAAF LHAYVSQAVS SGIRLGVAGQ RDGVAILAGL
     EDHITEVARR AAASTLDDLG SATVQADIAS LRHETQATRL FRS