ID   UREF_RHILW              Reviewed;         223 AA.
AC   B5ZMN6;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=Rleg2_3047;
OS   Rhizobium leguminosarum bv. trifolii (strain WSM2304).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=395492;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=WSM2304;
RX   PubMed=21304679; DOI=10.4056/sigs.44642;
RA   Reeve W., O'Hara G., Chain P., Ardley J., Brau L., Nandesena K., Tiwari R.,
RA   Malfatti S., Kiss H., Lapidus A., Copeland A., Nolan M., Land M.,
RA   Ivanova N., Mavromatis K., Markowitz V., Kyrpides N., Melino V., Denton M.,
RA   Yates R., Howieson J.;
RT   "Complete genome sequence of Rhizobium leguminosarum bv trifolii strain
RT   WSM2304, an effective microsymbiont of the South American clover Trifolium
RT   polymorphum.";
RL   Stand. Genomic Sci. 2:66-76(2010).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; CP001191; ACI56314.1; -; Genomic_DNA.
DR   RefSeq; WP_012558722.1; NC_011369.1.
DR   AlphaFoldDB; B5ZMN6; -.
DR   SMR; B5ZMN6; -.
DR   STRING; 395492.Rleg2_3047; -.
DR   EnsemblBacteria; ACI56314; ACI56314; Rleg2_3047.
DR   KEGG; rlt:Rleg2_3047; -.
DR   eggNOG; COG0830; Bacteria.
DR   HOGENOM; CLU_049215_2_0_5; -.
DR   OMA; TQYTRLF; -.
DR   OrthoDB; 1942614at2; -.
DR   Proteomes; UP000008330; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion.
FT   CHAIN           1..223
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_1000145138"
SQ   SEQUENCE   223 AA;  23423 MW;  5F71F927FC4AE378 CRC64;
     MTGDRELQAL LRLTAWLSPA FPVGSFAYSG GLERAVADGL VTDAVSLAAW IGTLIGYGSV
     WNDAVLLAES HRWQAEPARL FEIAALAEAL AGSRERHQET MLLGDAFLTA ARAWPDGVFE
     RLPDKAAYPV AVGAVTGAHG IGPEKALAVF LHAYASQAVS SGIRLGVTGQ RDGVAVLAGL
     EECITEVARR AAASTLDDLG SATVQADIAG LRHETQATRL FRS