ID   CADC_ALKPO              Reviewed;         122 AA.
AC   P30339; D3G1Y5;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   15-JUN-2010, sequence version 2.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Cadmium resistance transcriptional regulatory protein CadC;
DE   AltName: Full=Cadmium efflux system accessory protein;
GN   Name=cadC; OrderedLocusNames=BpOF4_21829;
OS   Alkalihalophilus pseudofirmus (strain ATCC BAA-2126 / JCM 17055 / OF4)
OS   (Bacillus pseudofirmus).
OG   Plasmid pBpOF4-02.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalophilus.
OX   NCBI_TaxID=398511;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1321115; DOI=10.1128/jb.174.15.4878-4884.1992;
RA   Ivey D.M., Guffanti A.A., Shen Z., Kudyan N., Krulwich T.A.;
RT   "The cadC gene product of alkaliphilic Bacillus firmus OF4 partially
RT   restores Na+ resistance to an Escherichia coli strain lacking an Na+/H+
RT   antiporter (NhaA).";
RL   J. Bacteriol. 174:4878-4884(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-2126 / JCM 17055 / OF4; PLASMID=pBpOF4-02;
RX   PubMed=21951522; DOI=10.1111/j.1462-2920.2011.02591.x;
RA   Janto B., Ahmed A., Ito M., Liu J., Hicks D.B., Pagni S., Fackelmayer O.J.,
RA   Smith T.A., Earl J., Elbourne L.D., Hassan K., Paulsen I.T., Kolsto A.B.,
RA   Tourasse N.J., Ehrlich G.D., Boissy R., Ivey D.M., Li G., Xue Y., Ma Y.,
RA   Hu F.Z., Krulwich T.A.;
RT   "Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that
RT   support the ability to grow in an external pH range from 7.5 to 11.4.";
RL   Environ. Microbiol. 13:3289-3309(2011).
CC   -!- FUNCTION: Metal-binding repressor for the cad operon. Involved in
CC       resistance to heavy metals, such as cadmium, bismuth, zinc or lead.
CC       Metal binding causes the repressor to dissociate from the DNA (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M90750; AAA22857.1; -; Genomic_DNA.
DR   EMBL; CP001880; ADC52361.1; -; Genomic_DNA.
DR   RefSeq; WP_003322301.1; NC_013793.1.
DR   AlphaFoldDB; P30339; -.
DR   SMR; P30339; -.
DR   EnsemblBacteria; ADC52361; ADC52361; BpOF4_21829.
DR   KEGG; bpf:BpOF4_21829; -.
DR   HOGENOM; CLU_097806_7_3_9; -.
DR   OMA; MNTCIRV; -.
DR   OrthoDB; 1772343at2; -.
DR   Proteomes; UP000001544; Plasmid pBpOF4-02.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0046686; P:response to cadmium ion; IEA:UniProtKB-KW.
DR   CDD; cd00090; HTH_ARSR; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011991; ArsR-like_HTH.
DR   InterPro; IPR018334; ArsR_HTH.
DR   InterPro; IPR001845; HTH_ArsR_DNA-bd_dom.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF01022; HTH_5; 1.
DR   PRINTS; PR00778; HTHARSR.
DR   SMART; SM00418; HTH_ARSR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS00846; HTH_ARSR_1; 1.
DR   PROSITE; PS50987; HTH_ARSR_2; 1.
PE   3: Inferred from homology;
KW   Cadmium; Cadmium resistance; DNA-binding; Metal-binding; Plasmid;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..122
FT                   /note="Cadmium resistance transcriptional regulatory
FT                   protein CadC"
FT                   /id="PRO_0000160616"
FT   DOMAIN          24..119
FT                   /note="HTH arsR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   DNA_BIND        59..78
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   BINDING         7
FT                   /ligand="Cd(2+)"
FT                   /ligand_id="ChEBI:CHEBI:48775"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   BINDING         11
FT                   /ligand="Cd(2+)"
FT                   /ligand_id="ChEBI:CHEBI:48775"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   BINDING         58
FT                   /ligand="Cd(2+)"
FT                   /ligand_id="ChEBI:CHEBI:48775"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   BINDING         60
FT                   /ligand="Cd(2+)"
FT                   /ligand_id="ChEBI:CHEBI:48775"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   CONFLICT        57
FT                   /note="L -> S (in Ref. 1; AAA22857)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        70
FT                   /note="V -> A (in Ref. 1; AAA22857)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   122 AA;  14033 MW;  B5ED485FCEF3ADBE CRC64;
     MNKKDTCEIF CYDEEKVNRI QGDLKTIDIV SVAQMLKAIA DENRAKITYA LCQDEELCVC
     DIANIIGITV ANASHHLRTL HKQGIVRYRK EGKLAFYSLD DEHIRQIMMI VLEHKKEVNV
     NV