UREF_RHOPT
ID UREF_RHOPT Reviewed; 240 AA.
AC B3QGJ8;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 02-SEP-2008, sequence version 1.
DT 03-AUG-2022, entry version 55.
DE RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=Rpal_4179;
OS Rhodopseudomonas palustris (strain TIE-1).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Bradyrhizobiaceae; Rhodopseudomonas.
OX NCBI_TaxID=395960;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TIE-1;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Emerson D.,
RA Newman D.K., Roden E., Richardson P.;
RT "Complete sequence of Rhodopseudomonas palustris TIE-1.";
RL Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Required for maturation of urease via the functional
CC incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC Rule:MF_01385}.
CC -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC hydrolysis-dependent molecular chaperone, activating the urease
CC apoprotein by helping to assemble the nickel containing metallocenter
CC of UreC. The UreE protein probably delivers the nickel.
CC {ECO:0000255|HAMAP-Rule:MF_01385}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC Rule:MF_01385}.
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DR EMBL; CP001096; ACF02675.1; -; Genomic_DNA.
DR RefSeq; WP_012497078.1; NC_011004.1.
DR AlphaFoldDB; B3QGJ8; -.
DR SMR; B3QGJ8; -.
DR EnsemblBacteria; ACF02675; ACF02675; Rpal_4179.
DR KEGG; rpt:Rpal_4179; -.
DR HOGENOM; CLU_049215_2_0_5; -.
DR OMA; TQYTRLF; -.
DR OrthoDB; 1942614at2; -.
DR BioCyc; RPAL395960:RPAL_RS20675-MON; -.
DR Proteomes; UP000001725; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR Gene3D; 1.10.4190.10; -; 1.
DR HAMAP; MF_01385; UreF; 1.
DR InterPro; IPR002639; UreF.
DR InterPro; IPR038277; UreF_sf.
DR Pfam; PF01730; UreF; 1.
DR PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE 3: Inferred from homology;
KW Chaperone; Cytoplasm; Nickel insertion.
FT CHAIN 1..240
FT /note="Urease accessory protein UreF"
FT /id="PRO_1000145139"
SQ SEQUENCE 240 AA; 25255 MW; 58C3B9301CF58693 CRC64;
MSTEPEASAQ PLPAQQSAAL FRLMTWLSPA FPVGAFSYSS GIEWAVEAGD VEGAASLRDW
LDAMLQHGAG FCDAVLLCHA YRATERHDDA GLLAVAELAA ALVTSAERQL ETLSQGRAFI
EIARKAWNSD GLGRAVAACG EAIAYPVAVG VVSASHGVPL PATLHGFLHA VTSNWISAGA
RLIPLGQTDS QRVLAALEPS VIATGTRALT ASLDDLGTAT FRADLASLRH ETQYTRLFRS