ID   UREF_SPOPA              Reviewed;         202 AA.
AC   Q45345; Q93KP9;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   22-JUL-2008, sequence version 2.
DT   25-MAY-2022, entry version 51.
DE   RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385};
OS   Sporosarcina pasteurii (Bacillus pasteurii).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Planococcaceae; Sporosarcina.
OX   NCBI_TaxID=1474;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 11859 / DSM 33 / NCIB 8841 / NCTC 4822;
RX   PubMed=12072968; DOI=10.1007/s00775-002-0341-7;
RA   Ciurli S., Safarov N., Miletti S., Dikiy A., Christensen S.K.,
RA   Kornetzky K., Bryant D.A., Vandenberghe I., Devreese B., Samyn B.,
RA   Remaut H., Van Beeumen J.;
RT   "Molecular characterization of Bacillus pasteurii UreE, a metal-binding
RT   chaperone for the assembly of the urease active site.";
RL   J. Biol. Inorg. Chem. 7:623-631(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 33-159.
RC   STRAIN=ATCC 11859 / DSM 33 / NCIB 8841 / NCTC 4822;
RA   You J.-H., Kim J.-G., Song B.-H., Lee M.-H., Kim S.-D.;
RT   "Genetic organization and nucleotide sequence of the ure gene cluster in
RT   Bacillus pasteurii.";
RL   Mol. Cells 5:359-369(1995).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; AF361945; AAK43719.1; -; Genomic_DNA.
DR   EMBL; U29368; AAA73988.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q45345; -.
DR   SMR; Q45345; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion.
FT   CHAIN           1..202
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_0000067644"
FT   CONFLICT        33..38
FT                   /note="AIIAQE -> MQLSHK (in Ref. 2; AAA73988)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        124..159
FT                   /note="TSLETFLYSSITSLVQNGVRAIPLGQNSGVQTIFSL -> HRLNIPIFINNI
FT                   SCSKRCSCNSAWSK (in Ref. 2; AAA73988)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   202 AA;  22967 MW;  5637E67384C113BC CRC64;
     METYIQESDI SNEDDLKAFC DMYLRQNLAS TDAIIAQEAY RLAKENDLQG LIRLENICHA
     IKLSPETRKG SMMMGRQFLQ TVQPLNNSEL FTIWCEKLKN KEIKSHYPVV YGIYTAMLGV
     DLRTSLETFL YSSITSLVQN GVRAIPLGQN SGVQTIFSLL PVIQETTSRV MTLDLEHLDN
     NSIGLEIASM KHEFLHSRLF IS