ID   UREF_STAA1              Reviewed;         229 AA.
AC   A7X5M5;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=SAHV_2276;
OS   Staphylococcus aureus (strain Mu3 / ATCC 700698).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=418127;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Mu3 / ATCC 700698;
RX   PubMed=17954695; DOI=10.1128/aac.00534-07;
RA   Neoh H.-M., Cui L., Yuzawa H., Takeuchi F., Matsuo M., Hiramatsu K.;
RT   "Mutated response regulator graR is responsible for phenotypic conversion
RT   of Staphylococcus aureus from heterogeneous vancomycin-intermediate
RT   resistance to vancomycin-intermediate resistance.";
RL   Antimicrob. Agents Chemother. 52:45-53(2008).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; AP009324; BAF79159.1; -; Genomic_DNA.
DR   RefSeq; WP_000565254.1; NC_009782.1.
DR   AlphaFoldDB; A7X5M5; -.
DR   SMR; A7X5M5; -.
DR   KEGG; saw:SAHV_2276; -.
DR   HOGENOM; CLU_049215_4_2_9; -.
DR   OMA; TQYTRLF; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion.
FT   CHAIN           1..229
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_0000344185"
SQ   SEQUENCE   229 AA;  26552 MW;  D20A5E967E69E4E1 CRC64;
     MIDHTHLRLF QFCDSQFPTG AFSHSFGLET YIQRNIIHDD HTFIAWLKMF LQEQLTYSDG
     LAMRLVYDAL ENDDTQKVLH IDKLMFVQNL PKETRVGAKQ MGTRMVKLAL ELYNSPWIAW
     YHQQMQDKKA KLNPAICFTM LGHHLGVDIE TIIDYYLYQN VSSLTQNAVR AIPLGQTAGQ
     KIVTHMIPYI EETRKQIFEL KEADFGMTAP GLELNQMAHE NVNVRIFIS