ID   UREF_STAA3              Reviewed;         229 AA.
AC   Q2FEK1;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   OrderedLocusNames=SAUSA300_2242;
OS   Staphylococcus aureus (strain USA300).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=367830;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=USA300;
RX   PubMed=16517273; DOI=10.1016/s0140-6736(06)68231-7;
RA   Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G.,
RA   Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F.,
RA   Perdreau-Remington F.;
RT   "Complete genome sequence of USA300, an epidemic clone of community-
RT   acquired meticillin-resistant Staphylococcus aureus.";
RL   Lancet 367:731-739(2006).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; CP000255; ABD22468.1; -; Genomic_DNA.
DR   RefSeq; WP_000565255.1; NZ_CP027476.1.
DR   AlphaFoldDB; Q2FEK1; -.
DR   SMR; Q2FEK1; -.
DR   EnsemblBacteria; ABD22468; ABD22468; SAUSA300_2242.
DR   KEGG; saa:SAUSA300_2242; -.
DR   HOGENOM; CLU_049215_4_2_9; -.
DR   OMA; TQYTRLF; -.
DR   Proteomes; UP000001939; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion.
FT   CHAIN           1..229
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_0000344192"
SQ   SEQUENCE   229 AA;  26480 MW;  880A5E967E6AB2D4 CRC64;
     MIDHTHLRLF QFCDSQFPTG AFSHSFGLET YIQRNIIHDD HTFIAWLKMF LQEQLTYSDG
     LAMRLVYDAL ENDDTQKVLH IDKLMFVQNL PKETRVGAKQ MGTRMVKLAL ELYNSPWIAW
     YHQQMQDKKA KLNPAICFTM LGHHLGVDIE TIIDYYLYQN VSSLTQNAVR AIPLGQTAGQ
     KIVTHMIPYI EGTRKQIFEL KEADFGMTAP GLELNQMAHE NVNVRIFIS