ID   CADC_LACLL              Reviewed;         119 AA.
AC   P0A4U3; P94887;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Cadmium resistance transcriptional regulatory protein CadC;
DE   AltName: Full=Cadmium efflux system accessory protein;
GN   Name=cadC;
OS   Lactococcus lactis subsp. lactis (Streptococcus lactis).
OG   Plasmid pND302, and Plasmid pAH82.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=1360;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=M71; PLASMID=pND302;
RX   PubMed=9339465; DOI=10.1006/plas.1997.1301;
RA   Liu C.-Q., Khunajakr N., Chia L.G., Deng Y.-M., Charoenchai P., Dunn N.W.;
RT   "Genetic analysis of regions involved in replication and cadmium resistance
RT   of the plasmid pND302 from Lactococcus lactis.";
RL   Plasmid 38:79-90(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DPC220; PLASMID=pAH82;
RX   PubMed=10844674; DOI=10.1046/j.1365-2958.2000.01901.x;
RA   O'Sullivan D., Twomey D.P., Coffey A., Hill C., Fitzgerald G.F., Ross P.R.;
RT   "Novel type I restriction specificities through domain shuffling of HsdS
RT   subunits in Lactococcus lactis.";
RL   Mol. Microbiol. 36:866-875(2000).
CC   -!- FUNCTION: Metal-binding repressor for the cad operon. Involved in
CC       resistance to heavy metals, such as cadmium, bismuth, zinc or lead.
CC       Metal binding causes the repressor to dissociate from the DNA (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
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DR   EMBL; U78967; AAB37344.1; -; Genomic_DNA.
DR   EMBL; AF243383; AAF98301.1; -; Genomic_DNA.
DR   RefSeq; NP_862601.1; NC_004966.1.
DR   RefSeq; WP_003726380.1; NZ_WJUU01000003.1.
DR   RefSeq; YP_001174713.1; NC_009435.1.
DR   RefSeq; YP_001966470.1; NC_010901.1.
DR   RefSeq; YP_004761536.1; NC_015863.1.
DR   AlphaFoldDB; P0A4U3; -.
DR   SMR; P0A4U3; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0046686; P:response to cadmium ion; IEA:UniProtKB-KW.
DR   CDD; cd00090; HTH_ARSR; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011991; ArsR-like_HTH.
DR   InterPro; IPR018334; ArsR_HTH.
DR   InterPro; IPR001845; HTH_ArsR_DNA-bd_dom.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF01022; HTH_5; 1.
DR   PRINTS; PR00778; HTHARSR.
DR   SMART; SM00418; HTH_ARSR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS00846; HTH_ARSR_1; 1.
DR   PROSITE; PS50987; HTH_ARSR_2; 1.
PE   3: Inferred from homology;
KW   Cadmium; Cadmium resistance; DNA-binding; Metal-binding; Plasmid;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..119
FT                   /note="Cadmium resistance transcriptional regulatory
FT                   protein CadC"
FT                   /id="PRO_0000160617"
FT   DOMAIN          23..118
FT                   /note="HTH arsR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   DNA_BIND        58..77
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   BINDING         6
FT                   /ligand="Cd(2+)"
FT                   /ligand_id="ChEBI:CHEBI:48775"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   BINDING         10
FT                   /ligand="Cd(2+)"
FT                   /ligand_id="ChEBI:CHEBI:48775"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   BINDING         57
FT                   /ligand="Cd(2+)"
FT                   /ligand_id="ChEBI:CHEBI:48775"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   BINDING         59
FT                   /ligand="Cd(2+)"
FT                   /ligand_id="ChEBI:CHEBI:48775"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
SQ   SEQUENCE   119 AA;  13382 MW;  B34C01C21DC2C944 CRC64;
     MNNEICEITC IHEDKVNRAK SKLANFDTPS VSGFFKILSD ENRLKIVHAL VHEDELCVCD
     IANIIDASVA TTSHHLNSLK KLGVVDSHKD GKLVYYFIKN IKILNLMELG VNFKEEVLA