ID   UREF_STRT2              Reviewed;         237 AA.
AC   Q5M605;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=stu0285;
OS   Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=264199;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-250 / LMG 18311;
RX   PubMed=15543133; DOI=10.1038/nbt1034;
RA   Bolotin A., Quinquis B., Renault P., Sorokin A., Ehrlich S.D.,
RA   Kulakauskas S., Lapidus A., Goltsman E., Mazur M., Pusch G.D., Fonstein M.,
RA   Overbeek R., Kyprides N., Purnelle B., Prozzi D., Ngui K., Masuy D.,
RA   Hancy F., Burteau S., Boutry M., Delcour J., Goffeau A., Hols P.;
RT   "Complete sequence and comparative genome analysis of the dairy bacterium
RT   Streptococcus thermophilus.";
RL   Nat. Biotechnol. 22:1554-1558(2004).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; CP000023; AAV60007.1; -; Genomic_DNA.
DR   RefSeq; WP_002946398.1; NC_006448.1.
DR   AlphaFoldDB; Q5M605; -.
DR   SMR; Q5M605; -.
DR   STRING; 264199.stu0285; -.
DR   DNASU; 3164107; -.
DR   EnsemblBacteria; AAV60007; AAV60007; stu0285.
DR   GeneID; 66898210; -.
DR   KEGG; stl:stu0285; -.
DR   eggNOG; COG0830; Bacteria.
DR   HOGENOM; CLU_049215_4_2_9; -.
DR   OMA; TQYTRLF; -.
DR   Proteomes; UP000001170; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion; Reference proteome.
FT   CHAIN           1..237
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_1000145141"
SQ   SEQUENCE   237 AA;  26915 MW;  0BEA04C022908B88 CRC64;
     MNINPFANVS LQDYLEIVQI VDSTFPIGSF NHSFGMENYL REDTVTDDKG YEEWQEAYLA
     SQFKYGEGLV IKLVYDAMVT DNIDQVWYYD KVLTVSTQAR ETRQGTKMIA KQMLRLIQRL
     HAIPVLDDYQ SKIRKGVAFG NPAIVFALYV FNKGLGCNEA IALYGYSVIS TMVQNAVRAI
     PLGQFAGQEI VLRSFSQLEK MTQEIQELDA SYLGANTPGL ELAQMKHETQ VFRLFMS