ID   UREF_YEREN              Reviewed;         228 AA.
AC   P42870;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385};
OS   Yersinia enterocolitica.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=630;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=A2635 / Serotype O:8;
RX   PubMed=8045421; DOI=10.1016/0378-1119(94)90318-2;
RA   de Koning-Ward T.F., Ward A.C., Robins-Browne R.M.;
RT   "Characterisation of the urease-encoding gene complex of Yersinia
RT   enterocolitica.";
RL   Gene 145:25-32(1994).
RN   [2]
RP   ROLE IN VIRULENCE.
RC   STRAIN=W22703 / Serogroup O:9;
RX   PubMed=7558281; DOI=10.1128/iai.63.10.3790-3795.1995;
RA   de Koning-Ward T.F., Robins-Browne R.M.;
RT   "Contribution of urease to acid tolerance in Yersinia enterocolitica.";
RL   Infect. Immun. 63:3790-3795(1995).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- FUNCTION: Expression of the urease operon increases the likelihood of
CC       bacterial survival by contibuting to acid resistance in vitro and in
CC       vivo in BALB/c mice. Y.enterocolitica enters the body via an oral path
CC       and must survive the acidic stomach before being able to colonize the
CC       intestinal mucosa (PubMed:7558281). {ECO:0000269|PubMed:7558281}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
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DR   EMBL; L24101; AAA50998.1; -; Genomic_DNA.
DR   RefSeq; WP_005164256.1; NZ_UHIX01000001.1.
DR   AlphaFoldDB; P42870; -.
DR   SMR; P42870; -.
DR   GeneID; 67420529; -.
DR   OMA; HVRLFMN; -.
DR   OrthoDB; 1942614at2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion; Virulence.
FT   CHAIN           1..228
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_0000067657"
SQ   SEQUENCE   228 AA;  25041 MW;  0EB2536245EBFB34 CRC64;
     MNASDLIRIM QFGDSVLPVG AFTFSNGVES AIQTGVVRDV PTLKGFVLTA LKQAASCDGM
     GVVAAHRAVV ADDRDGIIRA DWAVNNRKLN EESRLMATRM GKKLAEMSIH VVEHPLISWW
     LEQIKNGNTA GTYPVTQAVV MAAQGIGQRE VVVMHQYGVA MTILSAAMRL MRVTHFDTQH
     ILFELNHDIE KFCDIAEIGD IDQMSSYVPI VDVLAAVHVK AHVRLFSN