ID   UREF_YERPE              Reviewed;         228 AA.
AC   Q9ZFR7; Q0WDM0;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   16-NOV-2001, sequence version 2.
DT   25-MAY-2022, entry version 117.
DE   RecName: Full=Urease accessory protein UreF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   Name=ureF {ECO:0000255|HAMAP-Rule:MF_01385};
GN   OrderedLocusNames=YPO2669, y1241, YP_2470;
OS   Yersinia pestis.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=632;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND LACK OF ROLE IN VIRULENCE.
RC   STRAIN=6/69M;
RX   PubMed=11119503; DOI=10.1128/iai.69.1.170-176.2001;
RA   Sebbane F., Devalckenaere A., Foulon J., Carniel E., Simonet M.;
RT   "Silencing and reactivation of urease in Yersinia pestis is determined by
RT   one G residue at a specific position in the ureD gene.";
RL   Infect. Immun. 69:170-176(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CO-92 / Biovar Orientalis;
RX   PubMed=11586360; DOI=10.1038/35097083;
RA   Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA   Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA   Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA   Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G., Feltwell T.,
RA   Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S.,
RA   Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J.,
RA   Stevens K., Whitehead S., Barrell B.G.;
RT   "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL   Nature 413:523-527(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KIM10+ / Biovar Mediaevalis;
RX   PubMed=12142430; DOI=10.1128/jb.184.16.4601-4611.2002;
RA   Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P.,
RA   Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D.,
RA   Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A.,
RA   Nilles M.L., Matson J.S., Blattner F.R., Perry R.D.;
RT   "Genome sequence of Yersinia pestis KIM.";
RL   J. Bacteriol. 184:4601-4611(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=91001 / Biovar Mediaevalis;
RX   PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA   Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA   Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L.,
RA   Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H.,
RA   Wang J., Huang P., Yang R.;
RT   "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT   avirulent to humans.";
RL   DNA Res. 11:179-197(2004).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC   -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01385}.
CC   -!- CAUTION: The last gene of this probable operon, ureD, gives rise to a
CC       truncated protein. Absence of ureD prevents expression of active
CC       urease. Correction of the mutation does not effect virulence in mice in
CC       any detectable fashion, suggesting urease is not important in the
CC       virulence of Y.pestis (PubMed:11119503). {ECO:0000305|PubMed:11119503}.
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DR   EMBL; AF095636; AAC78636.1; -; Genomic_DNA.
DR   EMBL; AL590842; CAL21288.1; -; Genomic_DNA.
DR   EMBL; AE009952; AAM84816.1; -; Genomic_DNA.
DR   EMBL; AE017042; AAS62670.1; -; Genomic_DNA.
DR   PIR; AE0325; AE0325.
DR   RefSeq; WP_002212231.1; NZ_WUCM01000006.1.
DR   RefSeq; YP_002347618.1; NC_003143.1.
DR   AlphaFoldDB; Q9ZFR7; -.
DR   SMR; Q9ZFR7; -.
DR   STRING; 214092.YPO2669; -.
DR   PaxDb; Q9ZFR7; -.
DR   DNASU; 1146188; -.
DR   EnsemblBacteria; AAM84816; AAM84816; y1241.
DR   EnsemblBacteria; AAS62670; AAS62670; YP_2470.
DR   GeneID; 57976025; -.
DR   KEGG; ype:YPO2669; -.
DR   KEGG; ypk:y1241; -.
DR   KEGG; ypm:YP_2470; -.
DR   PATRIC; fig|214092.21.peg.3103; -.
DR   eggNOG; COG0830; Bacteria.
DR   HOGENOM; CLU_049215_4_0_6; -.
DR   OMA; HVRLFMN; -.
DR   Proteomes; UP000000815; Chromosome.
DR   Proteomes; UP000001019; Chromosome.
DR   Proteomes; UP000002490; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.4190.10; -; 1.
DR   HAMAP; MF_01385; UreF; 1.
DR   InterPro; IPR002639; UreF.
DR   InterPro; IPR038277; UreF_sf.
DR   Pfam; PF01730; UreF; 1.
DR   PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion; Reference proteome.
FT   CHAIN           1..228
FT                   /note="Urease accessory protein UreF"
FT                   /id="PRO_0000067658"
FT   CONFLICT        70
FT                   /note="V -> M (in Ref. 1; AAC78636)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   228 AA;  25037 MW;  AB1631F780AD54AC CRC64;
     MNASDLIRIM QFGDSVLPVG AFTFSNGVES AIQTGIVHDV ATLKGFVLTA LKQAASCDGM
     GVVVAHRAVV ADDRDGIIRA DWAVNNRKLN EESRLMATRM GKKLAEMSIH VVEHPLISWW
     LEQIKNGNTA GTYPVTQAVV MAAQGIGQRE VVVMHQYGVA MTILSAAMRL MRVTHFDTQH
     ILFELNHDIE KFCDIAEIGD INQMSSYVPI VDVLAAVHVK AHVRLFSN