UREG_ECO57
ID UREG_ECO57 Reviewed; 205 AA.
AC Q8X9U4; Q7AFH2;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Urease accessory protein UreG {ECO:0000255|HAMAP-Rule:MF_01389};
GN Name=ureG1 {ECO:0000255|HAMAP-Rule:MF_01389};
GN OrderedLocusNames=Z1148, ECs1327;
GN and
GN Name=ureG2 {ECO:0000255|HAMAP-Rule:MF_01389}; OrderedLocusNames=Z1587;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
RN [3]
RP ABSENCE OF UREASE.
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=15470125; DOI=10.1099/mic.0.27280-0;
RA Nakano M., Iida T., Honda T.;
RT "Urease activity of enterohaemorrhagic Escherichia coli depends on a
RT specific one-base substitution in ureD.";
RL Microbiology 150:3483-3489(2004).
CC -!- FUNCTION: Facilitates the functional incorporation of the urease nickel
CC metallocenter. This process requires GTP hydrolysis, probably
CC effectuated by UreG. {ECO:0000255|HAMAP-Rule:MF_01389}.
CC -!- SUBUNIT: Homodimer. UreD, UreF and UreG form a complex that acts as a
CC GTP-hydrolysis-dependent molecular chaperone, activating the urease
CC apoprotein by helping to assemble the nickel containing metallocenter
CC of UreC. The UreE protein probably delivers the nickel.
CC {ECO:0000255|HAMAP-Rule:MF_01389}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01389}.
CC -!- SIMILARITY: Belongs to the SIMIBI class G3E GTPase family. UreG
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01389}.
CC -!- CAUTION: Neither O157 strain expresses urease due to a truncation of
CC ureD, the last gene of the probable operon. Urease activity is restored
CC in O157 / Sakai upon complementation with wild-type ureD.
CC {ECO:0000305}.
CC -!- CAUTION: This region of the chromosome is duplicated in strain O157:H7
CC / EDL933 but not in O157:H7 / Sakai. {ECO:0000305}.
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DR EMBL; AE005174; AAG55293.1; -; Genomic_DNA.
DR EMBL; AE005174; AAG55702.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB34750.1; -; Genomic_DNA.
DR PIR; A85604; A85604.
DR PIR; G90794; G90794.
DR RefSeq; NP_309354.1; NC_002695.1.
DR RefSeq; WP_001021389.1; NZ_SEKU01000027.1.
DR AlphaFoldDB; Q8X9U4; -.
DR SMR; Q8X9U4; -.
DR STRING; 155864.EDL933_1047; -.
DR EnsemblBacteria; AAG55293; AAG55293; Z1148.
DR EnsemblBacteria; AAG55702; AAG55702; Z1587.
DR EnsemblBacteria; BAB34750; BAB34750; ECs_1327.
DR GeneID; 912768; -.
DR KEGG; ece:Z1148; -.
DR KEGG; ece:Z1587; -.
DR KEGG; ecs:ECs_1327; -.
DR PATRIC; fig|386585.9.peg.1432; -.
DR eggNOG; COG0378; Bacteria.
DR HOGENOM; CLU_072144_1_0_6; -.
DR OMA; VDLTIYV; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01389; UreG; 1.
DR InterPro; IPR003495; CobW/HypB/UreG_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004400; UreG.
DR PANTHER; PTHR31715; PTHR31715; 1.
DR Pfam; PF02492; cobW; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00101; ureG; 1.
PE 3: Inferred from homology;
KW Chaperone; Cytoplasm; GTP-binding; Nickel insertion; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..205
FT /note="Urease accessory protein UreG"
FT /id="PRO_0000347390"
FT BINDING 14..21
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01389"
SQ SEQUENCE 205 AA; 22124 MW; 80126E5567972FB9 CRC64;
MNIIKQPLRV GVGGPVGSGK TALLEALCKS MRDTWQLAVV TNDIYTREDQ RILTEAGALE
AERIVGVETG GCPHTAIRED ASMNLAAVEA LSEKFGNLEL IFVESGGDNL SATFSPELAD
LTIYVIDVAE GEKIPRKGGP GITKSDFLVI NKTDLAPYVG ASLEVMERDT LRMRGERPWG
FTNLKSGEGL QNIIAFIEEQ GMLGK
