ID   UREG_HELHP              Reviewed;         200 AA.
AC   Q93PJ0; Q7BY99;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Urease accessory protein UreG {ECO:0000255|HAMAP-Rule:MF_01389};
GN   Name=ureG {ECO:0000255|HAMAP-Rule:MF_01389}; OrderedLocusNames=HH_0412;
OS   Helicobacter hepaticus (strain ATCC 51449 / 3B1).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=235279;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MU94-1;
RX   PubMed=11500473; DOI=10.1128/iai.69.9.5914-5920.2001;
RA   Beckwith C.S., McGee D.J., Mobley H.L.T., Riley L.K.;
RT   "Cloning, expression, and catalytic activity of Helicobacter hepaticus
RT   urease.";
RL   Infect. Immun. 69:5914-5920(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51449 / 3B1;
RX   PubMed=12810954; DOI=10.1073/pnas.1332093100;
RA   Suerbaum S., Josenhans C., Sterzenbach T., Drescher B., Brandt P., Bell M.,
RA   Droege M., Fartmann B., Fischer H.-P., Ge Z., Hoerster A., Holland R.,
RA   Klein K., Koenig J., Macko L., Mendz G.L., Nyakatura G., Schauer D.B.,
RA   Shen Z., Weber J., Frosch M., Fox J.G.;
RT   "The complete genome sequence of the carcinogenic bacterium Helicobacter
RT   hepaticus.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7901-7906(2003).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 51449 / 3B1;
RX   PubMed=17975083; DOI=10.1099/mic.0.2007/010520-0;
RA   Benoit S.L., Zbell A.L., Maier R.J.;
RT   "Nickel enzyme maturation in Helicobacter hepaticus: roles of accessory
RT   proteins in hydrogenase and urease activities.";
RL   Microbiology 153:3748-3756(2007).
CC   -!- FUNCTION: Facilitates the functional incorporation of the urease nickel
CC       metallocenter. This process requires GTP hydrolysis, probably
CC       effectuated by UreG. {ECO:0000255|HAMAP-Rule:MF_01389}.
CC   -!- SUBUNIT: Homodimer. UreH, UreF and UreG form a complex that acts as a
CC       GTP-hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01389}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01389}.
CC   -!- DISRUPTION PHENOTYPE: Cells missing this gene do not produce urease;
CC       addition of Ni(2+) to cell cultures does not compensate this loss of
CC       function. Normal amounts of apourease are produced by the cells. Has no
CC       effect on hydrogenase activity. {ECO:0000269|PubMed:17975083}.
CC   -!- SIMILARITY: Belongs to the SIMIBI class G3E GTPase family. UreG
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01389}.
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DR   EMBL; AF332656; AAK69203.1; -; Genomic_DNA.
DR   EMBL; AE017125; AAP77009.1; -; Genomic_DNA.
DR   RefSeq; WP_011115254.1; NC_004917.1.
DR   AlphaFoldDB; Q93PJ0; -.
DR   SMR; Q93PJ0; -.
DR   STRING; 235279.HH_0412; -.
DR   EnsemblBacteria; AAP77009; AAP77009; HH_0412.
DR   KEGG; hhe:HH_0412; -.
DR   eggNOG; COG0378; Bacteria.
DR   HOGENOM; CLU_072144_1_0_7; -.
DR   OMA; VDLTIYV; -.
DR   OrthoDB; 1134430at2; -.
DR   Proteomes; UP000002495; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01389; UreG; 1.
DR   InterPro; IPR003495; CobW/HypB/UreG_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR004400; UreG.
DR   PANTHER; PTHR31715; PTHR31715; 1.
DR   Pfam; PF02492; cobW; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00101; ureG; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; GTP-binding; Nickel insertion; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..200
FT                   /note="Urease accessory protein UreG"
FT                   /id="PRO_0000347394"
FT   BINDING         8..15
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01389"
SQ   SEQUENCE   200 AA;  22130 MW;  36A4AB054077566B CRC64;
     MVKIGICGPV GSGKTALIES LTRKMNEQYS LAVITNDIYT QEDAEFMSKN SVLPSERIIG
     VETGGCPHTA IREDASMNLE AVEEMQERFP DIEILFIESG GDNLSATFSP ELADFTIFVI
     DVAQGDKIPR KGGPGIMRSD LLIINKIDLA PYVGANLSIM ERDAKKMRED KPFLFTNIRA
     KEGIDAVVEW IKKYALLEDI