位置:首页 > 蛋白库 > UREG_HELPY
UREG_HELPY
ID   UREG_HELPY              Reviewed;         199 AA.
AC   Q09066;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=Urease accessory protein UreG {ECO:0000255|HAMAP-Rule:MF_01389};
GN   Name=ureG {ECO:0000255|HAMAP-Rule:MF_01389}; OrderedLocusNames=HP_0068;
OS   Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=85962;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RC   STRAIN=85P;
RX   PubMed=1313413; DOI=10.1128/jb.174.8.2466-2473.1992;
RA   Cussac V., Ferrero R.L., Labigne A.;
RT   "Expression of Helicobacter pylori urease genes in Escherichia coli grown
RT   under nitrogen-limiting conditions.";
RL   J. Bacteriol. 174:2466-2473(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700392 / 26695;
RX   PubMed=9252185; DOI=10.1038/41483;
RA   Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA   Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA   Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA   Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA   McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA   Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA   Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA   Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT   "The complete genome sequence of the gastric pathogen Helicobacter
RT   pylori.";
RL   Nature 388:539-547(1997).
RN   [3]
RP   MUTAGENESIS OF LYS-14.
RC   STRAIN=ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487;
RX   PubMed=14521881; DOI=10.1016/s0882-4010(03)00151-7;
RA   Mehta N., Benoit S., Maier R.J.;
RT   "Roles of conserved nucleotide-binding domains in accessory proteins, HypB
RT   and UreG, in the maturation of nickel-enzymes required for efficient
RT   Helicobacter pylori colonization.";
RL   Microb. Pathog. 35:229-234(2003).
RN   [4]
RP   REVIEW ON VIRULENCE OF H.PYLORI.
RX   PubMed=17313591; DOI=10.1111/j.1574-6968.2007.00648.x;
RA   Clyne M., Dolan B., Reeves E.P.;
RT   "Bacterial factors that mediate colonization of the stomach and virulence
RT   of Helicobacter pylori.";
RL   FEMS Microbiol. Lett. 268:135-143(2007).
CC   -!- FUNCTION: Facilitates the functional incorporation of the urease nickel
CC       metallocenter. This process requires GTP hydrolysis, probably
CC       effectuated by UreG. {ECO:0000255|HAMAP-Rule:MF_01389}.
CC   -!- SUBUNIT: Homodimer. UreH, UreF and UreG form a complex that acts as a
CC       GTP-hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01389}.
CC   -!- INTERACTION:
CC       Q09066; Q09064: ureE; NbExp=3; IntAct=EBI-7742750, EBI-7742396;
CC       Q09066; Q09065: ureF; NbExp=6; IntAct=EBI-7742750, EBI-7743673;
CC       Q09066; Q09066: ureG; NbExp=3; IntAct=EBI-7742750, EBI-7742750;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01389}.
CC   -!- DISRUPTION PHENOTYPE: Cells do not express urease.
CC       {ECO:0000269|PubMed:1313413}.
CC   -!- SIMILARITY: Belongs to the SIMIBI class G3E GTPase family. UreG
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01389}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M84338; AAA25025.1; -; Genomic_DNA.
DR   EMBL; AE000511; AAD07131.1; -; Genomic_DNA.
DR   PIR; D64528; D64528.
DR   RefSeq; NP_206868.1; NC_000915.1.
DR   RefSeq; WP_000238762.1; NC_018939.1.
DR   PDB; 4HI0; X-ray; 2.35 A; E/F=1-199.
DR   PDBsum; 4HI0; -.
DR   AlphaFoldDB; Q09066; -.
DR   SMR; Q09066; -.
DR   DIP; DIP-3131N; -.
DR   IntAct; Q09066; 3.
DR   MINT; Q09066; -.
DR   STRING; 85962.C694_00325; -.
DR   PaxDb; Q09066; -.
DR   PRIDE; Q09066; -.
DR   DNASU; 899102; -.
DR   EnsemblBacteria; AAD07131; AAD07131; HP_0068.
DR   GeneID; 66521325; -.
DR   KEGG; hpy:HP_0068; -.
DR   PATRIC; fig|85962.47.peg.71; -.
DR   eggNOG; COG0378; Bacteria.
DR   OMA; VDLTIYV; -.
DR   PhylomeDB; Q09066; -.
DR   BioCyc; MetaCyc:HP_RS00345-MON; -.
DR   Proteomes; UP000000429; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01389; UreG; 1.
DR   InterPro; IPR003495; CobW/HypB/UreG_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR004400; UreG.
DR   PANTHER; PTHR31715; PTHR31715; 1.
DR   Pfam; PF02492; cobW; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00101; ureG; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Chaperone; Cytoplasm; GTP-binding; Nickel insertion;
KW   Nucleotide-binding; Reference proteome; Virulence.
FT   CHAIN           1..199
FT                   /note="Urease accessory protein UreG"
FT                   /id="PRO_0000067667"
FT   BINDING         8..15
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01389"
FT   MUTAGEN         14
FT                   /note="K->A: No urease activity, no effect on hydrogenase
FT                   activity. Normal amounts of apourease and UreG protein are
FT                   produced."
FT                   /evidence="ECO:0000269|PubMed:14521881"
FT   CONFLICT        102
FT                   /note="D -> S (in Ref. 1; AAA25025)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        167..176
FT                   /note="MRGEKPFIFT -> IAAKSPLFLP (in Ref. 1; AAA25025)"
FT                   /evidence="ECO:0000305"
FT   STRAND          2..7
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   HELIX           14..25
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   TURN            26..28
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   STRAND          31..35
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   HELIX           41..51
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   HELIX           55..57
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   STRAND          58..60
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   HELIX           67..71
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   HELIX           76..88
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   STRAND          94..98
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   TURN            110..112
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   STRAND          114..121
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   TURN            122..124
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   HELIX           128..131
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   HELIX           134..137
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   STRAND          140..145
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   HELIX           147..149
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   HELIX           150..153
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   HELIX           157..168
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   STRAND          173..175
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   TURN            178..181
FT                   /evidence="ECO:0007829|PDB:4HI0"
FT   HELIX           184..194
FT                   /evidence="ECO:0007829|PDB:4HI0"
SQ   SEQUENCE   199 AA;  21955 MW;  A23E448EB6A208B1 CRC64;
     MVKIGVCGPV GSGKTALIEA LTRHMSKDYD MAVITNDIYT KEDAEFMCKN SVMPRERIIG
     VETGGCPHTA IREDASMNLE AVEEMHGRFP NLELLLIESG GDNLSATFNP ELADFTIFVI
     DVAEGDKIPR KGGPGITRSD LLVINKIDLA PYVGADLKVM ERDSKKMRGE KPFIFTNIRA
     KEGLDDVIAW IKRNALLED
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024